GOLGA2/GM130 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

GOLGA2/GM130 Monoclonal Antibody for WB, IF, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat

Applications

WB, IF, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2A4F11

Cat no : 66662-1-PBS

Synonyms

Cell and organelle markers, GM130, GM130 autoantigen, GOLGA2, Golgi Marker, Golgin 95, Golgin subfamily A member 2

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Validation Data Gallery

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  WB analysis of HEK-293 using 66662-1-Ig (same clone as 66662-1-PBS)

  WB result of GOLGA2/GM130 antibody (66662-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GOLGA2/GM130 transfected HEK-293 cells. This data was developed using the same antibody clone with 66662-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66662-1-Ig (same clone as 66662-1-PBS)

  WB result of GOLGA2/GM130 antibody (66662-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GOLGA2/GM130 transfected HeLa cells. This data was developed using the same antibody clone with 66662-1-PBS in a different storage buffer formulation.

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  WB analysis using 66662-1-Ig (same clone as 66662-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66662-1-Ig (GOLGA2/GM130 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66662-1-PBS in a different storage buffer formulation.

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  WB analysis using 66662-1-Ig (same clone as 66662-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66662-1-Ig (GM130;GOLGA2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66662-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66662-1-Ig (same clone as 66662-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GOLGA2/GM130 antibody (66662-1-Ig, Clone: 2A4F11 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66662-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66662-1-PBS targets GOLGA2/GM130 in WB, IF, Indirect ELISA applications and shows reactivity with Human, Rat samples.

• Tested Reactivity: Human, Rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: GOLGA2/GM130 fusion protein Ag1848
• Full Name: golgi autoantigen, golgin subfamily a, 2
• Calculated Molecular Weight: 111 kDa
• Observed Molecular Weight: 130 kDa
• GenBank Accession Number: BC014188
• Gene Symbol: GOLGA2
• Gene ID (NCBI): 2801
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

GOLGA2, also known as GM130, is a 130 kDa cis-Golgi matrix protein which is one component of the detergent and salt resistant Golgi matrix. It is a peripheral membrane protein highly bound to Golgi membrane and localized mainly at the cytoplasmic face of cis-Golgi membrane. Together with its interacting partner proteins, including p115, giantin, GRASP65, and Rab GTPase, GOLGA2/GM130 is involved in the regulation of ER-to-Golgi transport and also in the maintenance of the Golgi structure. Emerging evidence suggest that the GOLGA2/GM130 has potential roles in the control of glycosylation, cell cycle progression, and higher order cell functions such as cell polarization and directed cell migration. (PMID: 20197635)