|Positive WB detected in||LNCaP cells, HEK-293 cells, HepG2 cells, Jurkat cells, HSC-T6 cells, HeLa cells, K-562 cells, PC-12 cells|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Sample-dependent, check data in validation data gallery|
66662-1-Ig targets GOLGA2/GM130 in WB, ELISA applications and shows reactivity with Human, Rat samples.
|Tested Reactivity||Human, Rat|
|Cited Reactivity||human, rat|
|Host / Isotype||Mouse / IgG1|
|Immunogen||GOLGA2/GM130 fusion protein Ag1848|
|Full Name||golgi autoantigen, golgin subfamily a, 2|
|Calculated molecular weight||111 kDa|
|Observed molecular weight||130 kDa|
|GenBank accession number||BC014188|
|Gene ID (NCBI)||2801|
|Purification Method||Protein G purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
GOLGA2, also known as GM130, is a 130 kDa cis-Golgi matrix protein which is one component of the detergent and salt resistant Golgi matrix. It is a peripheral membrane protein highly bound to Golgi membrane and localized mainly at the cytoplasmic face of cis-Golgi membrane. Together with its interacting partner proteins, including p115, giantin, GRASP65, and Rab GTPase, GOLGA2/GM130 is involved in the regulation of ER-to-Golgi transport and also in the maintenance of the Golgi structure. Emerging evidence suggest that the GOLGA2/GM130 has potential roles in the control of glycosylation, cell cycle progression, and higher order cell functions such as cell polarization and directed cell migration. (PMID: 20197635)
Front Mol Biosci
Schwann Cell-Derived Exosomes Induce the Differentiation of Human Adipose-Derived Stem Cells Into Schwann Cells.
CD36 promotes de novo lipogenesis in hepatocytes through INSIG2-dependent SREBP1 processing.
J Extracell Vesicles
Extracellular vesicle-mediated delivery of circDYM alleviates CUS-induced depressive-like behaviours.