GOLGA2/GM130 Monoclonal antibody

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• KD/KO Validated

GOLGA2/GM130 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, rat and More (1)

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

12

CloneNo.

2A4F11

Cat no : 66662-1-Ig

Synonyms

GM130, GOLGA2, GM130;GOLGA2, GM130 autoantigen, 2A4F11

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Validation Data Gallery

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  WB analysis of HEK-293 using 66662-1-Ig

  WB result of GOLGA2/GM130 antibody (66662-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GOLGA2/GM130 transfected HEK-293 cells.

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  WB analysis of HeLa using 66662-1-Ig

  WB result of GOLGA2/GM130 antibody (66662-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GOLGA2/GM130 transfected HeLa cells.

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  WB analysis using 66662-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66662-1-Ig (GOLGA2/GM130 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 66662-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66662-1-Ig (GM130;GOLGA2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 66662-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GOLGA2/GM130 antibody (66662-1-Ig, Clone: 2A4F11 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: LNCaP cells, HEK-293 cells, HeLa cells, HepG2 cells, Jurkat cells, HSC-T6 cells, K-562 cells, PC-12 cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 9 publications below
• IF: See 3 publications below

Product Information

66662-1-Ig targets GOLGA2/GM130 in WB, IF/ICC, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: GOLGA2/GM130 fusion protein Ag1848
• Full Name: golgi autoantigen, golgin subfamily a, 2
• Calculated Molecular Weight: 111 kDa
• Observed Molecular Weight: 130 kDa
• GenBank Accession Number: BC014188
• Gene Symbol: GM130
• Gene ID (NCBI): 2801
• RRID: AB_2882017
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GOLGA2, also known as GM130, is a 130 kDa cis-Golgi matrix protein which is one component of the detergent and salt resistant Golgi matrix. It is a peripheral membrane protein highly bound to Golgi membrane and localized mainly at the cytoplasmic face of cis-Golgi membrane. Together with its interacting partner proteins, including p115, giantin, GRASP65, and Rab GTPase, GOLGA2/GM130 is involved in the regulation of ER-to-Golgi transport and also in the maintenance of the Golgi structure. Emerging evidence suggest that the GOLGA2/GM130 has potential roles in the control of glycosylation, cell cycle progression, and higher order cell functions such as cell polarization and directed cell migration. (PMID: 20197635)

Publications

Species	Application	Title
human	WB	J Extracell Vesicles; Extracellular vesicle-mediated delivery of circDYM alleviates CUS-induced depressive-like behaviours.; Authors - Xiaoyu Yu
human	WB	Mol Metab; CD36 promotes de novo lipogenesis in hepatocytes through INSIG2-dependent SREBP1 processing.; Authors - Han Zeng
human,rat	WB	Front Mol Biosci; Schwann Cell-Derived Exosomes Induce the Differentiation of Human Adipose-Derived Stem Cells Into Schwann Cells.; Authors - Nan Zhou
human	WB	Anticancer Drugs; Exosomal circRNA Scm-like with four malignant brain tumor domains 2 (circ-SFMBT2) enhances the docetaxel resistance of prostate cancer via the microRNA-136-5p/tribbles homolog 1 pathway; Authors - Xiaoliang Tan
human	WB	Nat Commun; Identification of indocyanine green as a STT3B inhibitor against mushroom α-amanitin cytotoxicity; Authors - Bei Wang
human	WB	J Mater Sci Mater Med; Enhanced therapeutic effects of mesenchymal stem cell-derived extracellular vesicles within chitosan hydrogel in the treatment of diabetic foot ulcers; Authors - Shuangshuang Yang