GNA12 Recombinant antibody, PBS Only (Capture)

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  WB analysis using 83263-4-RR (same clone as 83263-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83263-4-RR (GNA12 antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83263-4-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00276-1

  Cytometric bead array standard curve ofMP00276-1, GNA12 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83263-4-PBS. Detection antibody: 83263-3-PBS. Standard: Ag35068. Range: 0.625-20 ng/mL.

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  Cytometric bead array standard curve of MP00276-3

  Cytometric bead array standard curve ofMP00276-3, GNA12 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83263-4-PBS. Detection antibody: 83263-1-PBS. Standard: Ag35068. Range: 0.625-20 ng/mL.

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  Affinity and Kinetic Characterization of 83263-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83263-4-RR against Human GNA12 were performed. The affinity constant is 0.135 nM.

GNA12 Uni-rAb^TM Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA

Cat No. 83263-4-PBS

Clone No.240050E11

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Cytometric bead array, Indirect ELISA

Synonyms

GNA 12, gep, G protein subunit alpha 12, G alpha-12, G alpha 12

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Product Information

83263-4-PBS targets GNA12 as part of a matched antibody pair:

MP00276-1: 83263-4-PBS capture and 83263-3-PBS detection (validated in Cytometric bead array)

MP00276-3: 83263-4-PBS capture and 83263-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: GNA12 fusion protein Ag35068
• Full Name: guanine nucleotide binding protein (G protein) alpha 12
• GenBank Accession Number: BC087537
• Gene Symbol: GNA12
• Gene ID (NCBI): 2768
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q03113
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.