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GNA12 Recombinant antibody

GNA12 Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse , rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240050E11

Cat no : 83263-4-RR

Synonyms

G alpha 12, G protein subunit alpha 12, gep, GNA12, NNX3, RMP

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GNA12 Recombinant antibody (83263-4-RR)

    WB analysis using 83263-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83263-4-RR (GNA12 antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours.

  • Affinity experiments of 83263-4-RR

    Affinity experiments of 83263-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83263-4-RR against Human GNA12 were performed. The affinity constant is 0.135 nM.

Tested Applications

Positive WB detected inMCF-7 cells, HeLa cells, mouse pancreas tissue, rat pancreas tissue, HepG2 cells, HEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:14000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83263-4-RR targets GNA12 in WB, ELISA applications and shows reactivity with Human, mouse , rat samples.

Tested Reactivity Human, mouse , rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen GNA12 fusion protein Ag35068
Full Name guanine nucleotide binding protein (G protein) alpha 12
Observed Molecular Weight41 kDa
GenBank Accession NumberBC087537
Gene Symbol GNA12
Gene ID (NCBI) 2768
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for GNA12 antibody 83263-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols