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GLUT1 Recombinant antibody, PBS Only

GLUT1 Uni-rAbTM Recombinant Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

6F14

Cat no : 81463-1-PBS

Synonyms

SLC2A1, SLC2A1,GLUT1, GLUT-1, 6F14

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GLUT1 Recombinant antibody (81463-1-RR)

    WB analysis using 81463-1-RR (same clone as 81463-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81463-1-RR (SLC2A1,GLUT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81463-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using GLUT1 Recombinant antibody (81463-1-RR)

    IHC staining of human skin cancer using 81463-1-RR (same clone as 81463-1-PBS)

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 81463-1-RR (GLUT1 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81463-1-PBS in a different storage buffer formulation.

Product Information

81463-1-PBS targets GLUT1 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen GLUT1 fusion protein Ag16282
Full Name solute carrier family 2 (facilitated glucose transporter), member 1
Calculated Molecular Weight 492 aa, 54 kDa
Observed Molecular Weight 45-55 kDa
GenBank Accession NumberBC121804
Gene Symbol GLUT1
Gene ID (NCBI) 6513
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

GLUT1, also known as SLC2A1, is a ubiquitously expressed glucose transporter and is responsible for the basal level of glucose uptake in most cell types. Human erythrocytes express the highest level of GLUT1. Defects in SLC2A1 are the cause of GLUT1 deficiency syndrome type 1 and type 2. High expression of GLUT1 has been reported to be a reliable immunohistochemical marker for juvenile hemangiomas. GLUT1 protein may appear as two or more distinct forms among 43 kDa to 55 kDa due to the different glycosylation states. And the conversion of the highly glycosylated form of GLUT1 to a less glycosylated form has been reported to correlate with differentiation (PMID: 8263524, 23302780).