GLUT1 Recombinant antibody

GLUT1 Uni-rAb^TM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

CloneNo.

6F14

Cat no : 81463-1-RR

Synonyms

SLC2A1, SLC2A1,GLUT1, GLUT-1, 6F14

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Validation Data Gallery

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  WB analysis using 81463-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 81463-1-RR (SLC2A1,GLUT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human skin cancer using 81463-1-RR

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 81463-1-RR (GLUT1 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: unboiled HEK-293 cells, HeLa cells (incubated at 37°C)
• Positive IHC detected in: human skin cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

For optimal WB detection with 81463-1-RR, we recommend to avoid boiling the sample after lysis.

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 1 publications below

Product Information

81463-1-RR targets GLUT1 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: GLUT1 fusion protein Ag16282
• Full Name: solute carrier family 2 (facilitated glucose transporter), member 1
• Calculated Molecular Weight: 492 aa, 54 kDa
• Observed Molecular Weight: 45-55 kDa
• GenBank Accession Number: BC121804
• Gene Symbol: GLUT1
• Gene ID (NCBI): 6513
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GLUT1, also known as SLC2A1, is a ubiquitously expressed glucose transporter and is responsible for the basal level of glucose uptake in most cell types. Human erythrocytes express the highest level of GLUT1. Defects in SLC2A1 are the cause of GLUT1 deficiency syndrome type 1 and type 2. High expression of GLUT1 has been reported to be a reliable immunohistochemical marker for juvenile hemangiomas. GLUT1 protein may appear as two or more distinct forms among 43 kDa to 55 kDa due to the different glycosylation states. And the conversion of the highly glycosylated form of GLUT1 to a less glycosylated form has been reported to correlate with differentiation (PMID: 8263524, 23302780).

Publications

Species	Application	Title
human	WB	Br J Cancer; Relationship between the expression of PD-L1 and 18F-FDG uptake in pancreatic ductal adenocarcinoma; Authors - Jiajin Li
human,mouse	WB,IHC	Cell Death Dis; Scutellarin activates IDH1 to exert antitumor effects in hepatocellular carcinoma progression; Authors - Zhao Cui