CoraLite®594-conjugated GFP tag Polyclonal antibody

GFP tag Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

aequorea victoria, recombinant protein

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

Cat no : CL594-50430

Synonyms

eGFP, GFP, green, GFP-tag, eYFP



Tested Applications

Positive IF/ICC detected inTransfected HEK-293 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-50430 targets GFP tag in IF/ICC applications and shows reactivity with aequorea victoria, recombinant protein samples.

Tested Reactivity aequorea victoria, recombinant protein
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GFP tag fusion protein Ag2128
Full Name GFP tag
Calculated Molecular Weight 26 kDa
GenBank Accession NumberM62653
Gene Symbol
Gene ID (NCBI)
RRIDAB_2919882
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Green Fluorescent Proteins (GFPs) encompass a diverse range of proteins carrying a green chromophore, originating from various species and forming different protein lineages. 

Wildtype GFP consists of 238 amino acid residues (26.9 kDa). GFP was first identified in the jellyfish Aequorea victoria. It emits green light with a peak wavelength of 509 nm upon excitation by blue light at 395 nm.

When fused with other proteins, GFP serves as a versatile reporter protein e.g. for quantifying expression levels or facilitates visualization of subcellular localization through fluorescence microscopy.

This antibody is a rabbit polyclonal antibody, generated against the full-length eGFP protein. It exhibits reactivity towards variants of Aequorea victoria GFP, including S65T-GFP, RS-GFP, YFP, CFP, and eGFP.

This antibody is labeled with CoraLite Plus 594.


Protocols

Product Specific Protocols
IF protocol for CL594 GFP tag antibody CL594-50430Download protocol
Standard Protocols
Click here to view our Standard Protocols