CoraLite®594-conjugated GFP tag Polyclonal antibody

GFP tag Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

aequorea victoria, recombinant protein

Applications

WB, IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

Publications

1

Cat no : CL594-50430

Synonyms

eGFP, GFP, green, GFP-tag, eYFP

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Validation Data Gallery

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  IF Staining of Transfected HEK-293 using CL594-50430

  Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using CoraLite®594 GFP tag antibody (CL594-50430) at dilution of 1:200.

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Tested Applications

• Positive IF/ICC detected in: Transfected HEK-293 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

CL594-50430 targets GFP tag in WB, IF/ICC applications and shows reactivity with aequorea victoria, recombinant protein samples.

• Tested Reactivity: aequorea victoria, recombinant protein
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GFP tag fusion protein Ag2128
• Full Name: GFP tag
• Calculated Molecular Weight: 26 kDa
• GenBank Accession Number: M62653
• RRID: AB_2919882
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Green Fluorescent Proteins (GFPs) encompass a diverse range of proteins carrying a green chromophore, originating from various species and forming different protein lineages. 

Wildtype GFP consists of 238 amino acid residues (26.9 kDa). GFP was first identified in the jellyfish Aequorea victoria. It emits green light with a peak wavelength of 509 nm upon excitation by blue light at 395 nm.

When fused with other proteins, GFP serves as a versatile reporter protein e.g. for quantifying expression levels or facilitates visualization of subcellular localization through fluorescence microscopy.

This antibody is a rabbit polyclonal antibody, generated against the full-length eGFP protein. It exhibits reactivity towards variants of Aequorea victoria GFP, including S65T-GFP, RS-GFP, YFP, CFP, and eGFP.

This antibody is labeled with CoraLite Plus 594.

Publications

Species	Application	Title
	WB	Toxins (Basel); The Clostridium botulinum C2 Toxin Subunit C2IIa Delivers Enzymes with Positively Charged N-Termini into the Cytosol of Target Cells; Authors - Sebastian Heber