CoraLite® Plus 488-conjugated GFP tag Polyclonal antibody

GFP tag Polyclonal Antibody for IF-P

Host / Isotype

Rabbit / IgG

Reactivity

aequorea victoria, recombinant protein

Applications

IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-50430

Synonyms

eGFP, GFP, green, GFP-tag, eYFP

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Validation Data Gallery

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  IF Staining of mouse brain using CL488-50430

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using CoraLite® Plus 488 GFP tag antibody (CL488-50430) at dilution of 1:200, GFAP antibody (60190-1-Ig, Clone: 4B2E10, Magenta). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse brain using CL488-50430

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using CoraLite® Plus 488 GFP tag antibody (CL488-50430) at dilution of 1:200, GFAP antibody (60190-1-Ig, Clone: 4B2E10, Magenta). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IF-P detected in: mouse brain tissue

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-50430 targets GFP tag in IF-P applications and shows reactivity with aequorea victoria, recombinant protein samples.

• Tested Reactivity: aequorea victoria, recombinant protein
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GFP tag fusion protein Ag2128
• Full Name: GFP tag
• Calculated Molecular Weight: 26 kDa
• GenBank Accession Number: M62653
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Green Fluorescent Proteins (GFPs) encompass a diverse range of proteins carrying a green chromophore, originating from various species and forming different protein lineages. 

Wildtype GFP consists of 238 amino acid residues (26.9 kDa). GFP was first identified in the jellyfish Aequorea victoria. It emits green light with a peak wavelength of 509 nm upon excitation by blue light at 395 nm.

When fused with other proteins, GFP serves as a versatile reporter protein e.g. for quantifying expression levels or facilitates visualization of subcellular localization through fluorescence microscopy.

This antibody is a rabbit polyclonal antibody, generated against the full-length eGFP protein. It exhibits reactivity towards variants of Aequorea victoria GFP, including S65T-GFP, RS-GFP, YFP, CFP, and eGFP.

This antibody is labeled with CoraLite Plus 488.