CoraLite® Plus 647-conjugated GBP5 Polyclonal antibody

• Featured Product
• KD/KO Validated

GBP5 Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

Cat no : CL647-13220

Synonyms

Guanylate-binding protein 5, Guanine nucleotide-binding protein 5, GTP-binding protein 5, GBP-TA antigen, GBP-5

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Validation Data Gallery

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  FC experiment of MCF-7 using CL647-13220

  1x10^6 MCF-7 cells were intracellularly stained with 0.4 ug CoraLite® Plus 647-conjugated GBP5 Polyclonal antibody (CL647-13220)(red), or 0.4 ug CoraLite® Plus 647-conjugated Rabbit IgG control Rabbit PolyAb (CL647-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive FC (Intra) detected in: MCF-7 cells

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL647-13220 targets GBP5 in FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GBP5 fusion protein Ag4103
• Full Name: guanylate binding protein 5
• Calculated Molecular Weight: 586 aa, 67 kDa
• Observed Molecular Weight: 65 kDa, 55 kDa
• GenBank Accession Number: BC031639
• Gene Symbol: GBP5
• Gene ID (NCBI): 115362
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Guanylate binding protein (GBP) 5 belongs to the GBP family, which is involved in important cellular processes, including signal transduction, translation, vesicle trafficking, and exocytosis. GBP5 was reported to be a critical cellular factor in inflammasome assembly. Furthermore, GBP5 has been reported to induce inflammatory cytokines, including IL-1 beta and IL-18, through the activation of NLRP3 and AIM2. GBP5 encodes two isoforms: GBP-5a/b (67 kDa) and GBP-5ta (55 kDa)