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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 647-conjugated GBP2 Polyclonal antibody

GBP2 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

Cat no : CL647-11854

Synonyms

Guanylate-binding protein 2, Guanine nucleotide-binding protein 2, GTP-binding protein 2, GBP-2, GBP 2

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 647-conjugated GBP2 Polyclonal anti (CL647-11854)

    IF Staining of HeLa using CL647-11854

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 647 GBP2 antibody (CL647-11854) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HepG2 cells using CoraLite® Plus 647-conjugated GBP2 Polyclonal anti (CL647-11854)

    FC experiment of HepG2 using CL647-11854

    1x10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 647-conjugated GBP2 Polyclonal antibody (CL647-11854)(red), or 0.4 ug CoraLite® Plus 647-conjugated Rabbit IgG control Rabbit PolyAb (CL647-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-11854 targets GBP2 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GBP2 fusion protein Ag2425
Full Name guanylate binding protein 2, interferon-inducible
Calculated Molecular Weight 591 aa, 67 kDa
Observed Molecular Weight 67 kDa
GenBank Accession NumberBC022272
Gene Symbol GBP2
Gene ID (NCBI) 2634
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GBP2 is a member of the GTPase family and is crucial to host immunity against pathogens. GBP2 is also used as a marker of interferon (IFN) responsiveness, because it is one of the most highly expressed genes after IFN-γ stimulation. Increased GBP2 expression is also associated with a better prognosis in breast cancer and may have a role in T-cell defense against breast cancer. Interestingly, GBP2 expression in NIH/3T3 fibroblasts inhibited Rac activation and matrix metalloproteinase-9 expression, suggesting a possible role for GBP2 in regulating cancer metastasis. The molecular weight of GBP2 is 67 kDa. (PMID: 29072687, 35383115)

Protocols

Product Specific Protocols
IF protocol for CL Plus 647 GBP2 antibody CL647-11854Download protocol
Standard Protocols
Click here to view our Standard Protocols