GATA3 Recombinant antibody, PBS Only

GATA3 Uni-rAb^TM Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240684B8

Cat no : 83808-2-PBS

Synonyms

Trans-acting T-cell-specific transcription factor GATA-3, GATA-binding factor 3, GATA binding factor 3, 240684B8

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Validation Data Gallery

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  IF Staining of MCF-7 using 83808-2-RR (same clone as 83808-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using GATA3 antibody (83808-2-RR, Clone: 240684B8 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83808-2-PBS in a different storage buffer formulation.

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  FC experiment of Jurkat using 83808-2-RR (same clone as 83808-2-PBS)

  1x10^6 Jurkat cells were intracellularly stained with 0.25 ug GATA3 Recombinant antibody (83808-2-RR, Clone:240684B8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83808-2-PBS in a different storage buffer formulation.

• 

  FC experiment of U937 using 83808-2-RR (same clone as 83808-2-PBS)

  1x10^6 U937 were intracellularly stained with 0.25 ug GATA3 Recombinant antibody (83808-2-RR, Clone:240684B8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83808-2-PBS in a different storage buffer formulation.

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Product Information

The immunogen of 83808-2-PBS is GATA3 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: GATA3 fusion protein Ag0174
• Full Name: GATA binding protein 3
• Calculated Molecular Weight: 443 aa, 48 kDa
• GenBank Accession Number: BC003070
• Gene Symbol: GATA3
• Gene ID (NCBI): 2625
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

GATA3 is a transcriptional factor that binds to the enhancer elements of all 4 T-cell antigen receptor genes. GATA3 is highly expressed in naive, freshly activated cells and Th2 lineage cells and thought to be necessary and sufficient for Th2 cytokine gene expression. GATA3 and TBET interfer with each other to regulate gene expression. This is a rabbit polyclonal antibody raised against part of C-terminus of human GATA3.