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CoraLite® Plus 488-conjugated GATA2 Monoclonal antibody

GATA2 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2B5A1

Cat no : CL488-67722

Synonyms

NFE1B, GATA-binding protein 2, GATA binding protein 2, Endothelial transcription factor GATA-2, 2B5A1

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using CoraLite® Plus 488-conjugated GATA2 Monoclonal ant (CL488-67722)

    IF Staining of SH-SY5Y using CL488-67722

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using CoraLite® Plus 488 GATA2 antibody (CL488-67722, Clone: 2B5A1 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of K-562 cells using CoraLite® Plus 488-conjugated GATA2 Monoclonal ant (CL488-67722)

    FC experiment of K-562 using CL488-67722

    1X10^6 K-562 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human GATA2 (CL488-67722, Clone:2B5A1) (red), or 0.4 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (MOPC-21) (CL488-65124, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive IF/ICC detected inSH-SY5Y cells
Positive FC (Intra) detected inK-562 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67722 targets GATA2 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen GATA2 fusion protein Ag1557
Full Name GATA binding protein 2
Observed Molecular Weight50 kDa
GenBank Accession NumberBC018988
Gene Symbol GATA2
Gene ID (NCBI) 2624
RRIDAB_2919527
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GATA2 is a member of GATA family of transcription factors that contains zinc fingers in DNA binding domain. GATA2 acts as a transcriptinal activator that regulates endothelin-1 expression in endothelial cells. Together with GATA3, GATA2 regulates adipocyte differentiation through molecular control of the preadpocyte-adipocyte transition. It also affect the activity of Rho inhibitor p190RhoGAP that controls capillary network formation in vitro and retinal angiogenesis in vivo.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 GATA2 antibody CL488-67722Download protocol
FC protocol for CL Plus 488 GATA2 antibody CL488-67722Download protocol
Standard Protocols
Click here to view our Standard Protocols