GATA2 Monoclonal antibody

GATA2 Monoclonal Antibody for FC, IF, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, Rat

Applications

WB, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

2B5A1

Cat no : 67722-1-Ig

Synonyms

FLJ45948, GATA binding protein 2, GATA2, NFE1B



Tested Applications

Positive WB detected inHeLa cells, HUVEC cells, LNCaP cells, PC-12 cells, HepG2 cells, HEK-293 cells, K-562 cells, NIH/3T3 cells, bEnd.3 cells
Positive IF detected inL02 cells
Positive FC detected inK-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

67722-1-Ig targets GATA2 in WB, IF, FC, ELISA applications and shows reactivity with Human, mouse, Rat samples.

Tested Reactivity Human, mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen GATA2 fusion protein Ag1557
Full Name GATA binding protein 2
Observed molecular weight50 kDa
GenBank accession numberBC018988
Gene symbol GATA2
Gene ID (NCBI) 2624
RRIDAB_2882909
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GATA2 is a member of GATA family of transcription factors that contains zinc fingers in DNA binding domain. GATA2 acts as a transcriptinal activator that regulates endothelin-1 expression in endothelial cells. Together with GATA3, GATA2 regulates adipocyte differentiation through molecular control of the preadpocyte-adipocyte transition. It also affect the activity of Rho inhibitor p190RhoGAP that controls capillary network formation in vitro and retinal angiogenesis in vivo.

Protocols

Product Specific Protocols
WB protocol for GATA2 antibody 67722-1-IgDownload protocol
IF protocol for GATA2 antibody 67722-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols