GATA2 Monoclonal antibody, PBS Only

GATA2 Monoclonal Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

rat, mouse, human

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2B5A1

Cat no : 67722-1-PBS

Synonyms

NFE1B, GATA-binding protein 2, GATA binding protein 2, Endothelial transcription factor GATA-2, 2B5A1

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Validation Data Gallery

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  WB analysis using 67722-1-Ig (same clone as 67722-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67722-1-Ig (GATA2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67722-1-PBS in a different storage buffer formulation.

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  WB analysis of bEnd.3 using 67722-1-Ig (same clone as 67722-1-PBS)

  bEnd.3 cells were subjected to SDS PAGE followed by western blot with 67722-1-Ig (GATA2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67722-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 67722-1-Ig (same clone as 67722-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67722-1-Ig (GATA2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67722-1-PBS in a different storage buffer formulation.

• 

  WB analysis of PC-12 using 67722-1-Ig (same clone as 67722-1-PBS)

  PC-12 cells were subjected to SDS PAGE followed by western blot with 67722-1-Ig (GATA2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67722-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 67722-1-Ig (same clone as 67722-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 67722-1-Ig (GATA2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67722-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 67722-1-Ig (same clone as 67722-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67722-1-Ig (GATA2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67722-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 67722-1-Ig (same clone as 67722-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67722-1-Ig (GATA2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67722-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 67722-1-Ig (same clone as 67722-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 67722-1-Ig (GATA2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67722-1-PBS in a different storage buffer formulation.

• 

  WB analysis of LNCaP using 67722-1-Ig (same clone as 67722-1-PBS)

  LNCaP cells were subjected to SDS PAGE followed by western blot with 67722-1-Ig (GATA2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67722-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HUVEC using 67722-1-Ig (same clone as 67722-1-PBS)

  HUVEC cells were subjected to SDS PAGE followed by western blot with 67722-1-Ig (GATA2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67722-1-PBS in a different storage buffer formulation.

• 

  WB analysis of PC-12 using 67722-1-Ig (same clone as 67722-1-PBS)

  PC-12 cells were subjected to SDS PAGE followed by western blot with 67722-1-Ig (GATA2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67722-1-PBS in a different storage buffer formulation.

• 

  WB analysis of NIH/3T3 using 67722-1-Ig (same clone as 67722-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67722-1-Ig (GATA2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67722-1-PBS in a different storage buffer formulation.

• 

  IF Staining of SH-SY5Y using 67722-1-Ig (same clone as 67722-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using GATA2 antibody (67722-1-Ig, Clone: 2B5A1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67722-1-PBS in a different storage buffer formulation.

• 

  FC experiment of K-562 using 67722-1-Ig (same clone as 67722-1-PBS)

  1X10^6 K-562 cells were intracellularly stained with 0.5 ug Anti-Human GATA2 (67722-1-Ig, Clone:2B5A1) (red) labeled with FlexAble CoraLite® Plus 555 Antibody Labeling Kit for Mouse IgG1 (KFA022), or 0.5 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 67722-1-PBS in a different storage buffer formulation.

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Product Information

67722-1-PBS targets GATA2 in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with rat, mouse, human samples.

• Tested Reactivity: rat, mouse, human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: GATA2 fusion protein Ag1557
• Full Name: GATA binding protein 2
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC018988
• Gene Symbol: GATA2
• Gene ID (NCBI): 2624
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

GATA2 is a member of GATA family of transcription factors that contains zinc fingers in DNA binding domain. GATA2 acts as a transcriptinal activator that regulates endothelin-1 expression in endothelial cells. Together with GATA3, GATA2 regulates adipocyte differentiation through molecular control of the preadpocyte-adipocyte transition. It also affect the activity of Rho inhibitor p190RhoGAP that controls capillary network formation in vitro and retinal angiogenesis in vivo.