GATA2 Monoclonal antibody, PBS Only

GATA2 Monoclonal Antibody for WB, IF, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, Rat

Applications

WB, IF, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2B5A1

Cat no : 67722-1-PBS

Synonyms

FLJ45948, GATA binding protein 2, GATA2, NFE1B

---

Validation Data Gallery

• 

  WB analysis using 67722-1-Ig (same clone as 67722-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67722-1-Ig (GATA2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67722-1-PBS in a different storage buffer formulation.

• 

  WB analysis of bEnd.3 using 67722-1-Ig (same clone as 67722-1-PBS)

  bEnd.3 cells were subjected to SDS PAGE followed by western blot with 67722-1-Ig (GATA2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67722-1-PBS in a different storage buffer formulation.

• 

  WB analysis of NIH/3T3 using 67722-1-Ig (same clone as 67722-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67722-1-Ig (GATA2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67722-1-PBS in a different storage buffer formulation.

• 

  WB analysis of PC-12 using 67722-1-Ig (same clone as 67722-1-PBS)

  PC-12 cells were subjected to SDS PAGE followed by western blot with 67722-1-Ig (GATA2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67722-1-PBS in a different storage buffer formulation.

• 

  WB analysis of K-562 using 67722-1-Ig (same clone as 67722-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 67722-1-Ig (GATA2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67722-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 67722-1-Ig (same clone as 67722-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67722-1-Ig (GATA2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67722-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 67722-1-Ig (same clone as 67722-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67722-1-Ig (GATA2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67722-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 67722-1-Ig (same clone as 67722-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 67722-1-Ig (GATA2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67722-1-PBS in a different storage buffer formulation.

• 

  WB analysis of LNCaP using 67722-1-Ig (same clone as 67722-1-PBS)

  LNCaP cells were subjected to SDS PAGE followed by western blot with 67722-1-Ig (GATA2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67722-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HUVEC using 67722-1-Ig (same clone as 67722-1-PBS)

  HUVEC cells were subjected to SDS PAGE followed by western blot with 67722-1-Ig (GATA2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67722-1-PBS in a different storage buffer formulation.

• 

  WB analysis of PC-12 using 67722-1-Ig (same clone as 67722-1-PBS)

  PC-12 cells were subjected to SDS PAGE followed by western blot with 67722-1-Ig (GATA2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67722-1-PBS in a different storage buffer formulation.

• 

  WB analysis of NIH/3T3 using 67722-1-Ig (same clone as 67722-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67722-1-Ig (GATA2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67722-1-PBS in a different storage buffer formulation.

• 

  IF Staining of SH-SY5Y using 67722-1-Ig (same clone as 67722-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using GATA2 antibody (67722-1-Ig, Clone: 2B5A1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67722-1-PBS in a different storage buffer formulation.

---

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67722-1-PBS targets GATA2 in WB, IF, Indirect ELISA applications and shows reactivity with Human, mouse, Rat samples.

• Tested Reactivity: Human, mouse, Rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: GATA2 fusion protein Ag1557
• Full Name: GATA binding protein 2
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC018988
• Gene Symbol: GATA2
• Gene ID (NCBI): 2624
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

GATA2 is a member of GATA family of transcription factors that contains zinc fingers in DNA binding domain. GATA2 acts as a transcriptinal activator that regulates endothelin-1 expression in endothelial cells. Together with GATA3, GATA2 regulates adipocyte differentiation through molecular control of the preadpocyte-adipocyte transition. It also affect the activity of Rho inhibitor p190RhoGAP that controls capillary network formation in vitro and retinal angiogenesis in vivo.