GAR1 Recombinant antibody, PBS Only (Detector)

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  IHC staining of human skin cancer using 83577-1-RR (same clone as 83577-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 83577-1-RR (GAR1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83577-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin cancer using 83577-1-RR (same clone as 83577-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 83577-1-RR (GAR1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83577-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 83577-1-RR (same clone as 83577-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using GAR1 antibody (83577-1-RR, Clone: 240537C11 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83577-1-PBS in a different storage buffer formulation.

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  FC experiment of K562 using 83577-1-RR (same clone as 83577-1-PBS)

  1x10^6 K562 were intracellularly stained with 0.25 ug GAR1 Recombinant antibody (83577-1-RR, Clone:240537C11) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83577-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00568-1

  Cytometric bead array standard curve of MP00568-1, GAR1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83577-2-PBS. Detection antibody: 83577-1-PBS. Standard: Ag2282. Range: 0.781-100 ng/mL.

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  Cytometric bead array standard curve of MP00568-2

  Cytometric bead array standard curve of MP00568-2, GAR1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83577-3-PBS. Detection antibody: 83577-1-PBS. Standard: Ag2282. Range: 0.781-100 ng/mL.

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  Affinity and Kinetic Characterization of 83577-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83577-1-RR against Human GAR1 were performed. The affinity constant is below 1 pM.

GAR1 {ptg:Brand} Recombinant Antibody for IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Cat No. 83577-1-PBS

Clone No.240537C11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Synonyms

snoRNP protein GAR1, NOLA1, 240537C11

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Product Information

83577-1-PBS targets GAR1 as part of a matched antibody pair:

MP00568-1: 83577-2-PBS capture and 83577-1-PBS detection (validated in Cytometric bead array)

MP00568-2: 83577-3-PBS capture and 83577-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: GAR1 fusion protein Ag2282
• Full Name: GAR1 ribonucleoprotein homolog (yeast)
• Calculated Molecular Weight: 217 aa, 22 kDa
• GenBank Accession Number: BC003413
• Gene Symbol: GAR1
• Gene ID (NCBI): 54433
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9NY12
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

GAR1, other named NOLA1, is a subunit of H/ACA and telomerase. Thought is not required for H/ACA protein assembly, GAR1 is necessary for ribosome biogenesis and telomere. H/ACA involves in class specify the sites of uridine-to-pseudouridine. It contains a core domain that flanked by glycine- and arginine-rich(GAR) domains. GAR1 also required for correct processing or intranuclear trafficking of TERC, the RNA component of the telomerase reverse transciptase(TERT) holoenzyme.