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  • Immunohistochemistry (IHC) staining of human skin cancer tissue using GAR1 Recombinant antibody (83577-1-RR)

    IHC staining of human skin cancer using 83577-1-RR

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 83577-1-RR (GAR1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using GAR1 Recombinant antibody (83577-1-RR)

    IHC staining of human skin cancer using 83577-1-RR

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 83577-1-RR (GAR1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using GAR1 Recombinant antibody (83577-1-RR)

    IF Staining of HepG2 using 83577-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using GAR1 antibody (83577-1-RR, Clone: 240537C11 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of K562 using GAR1 Recombinant antibody (83577-1-RR)

    FC experiment of K562 using 83577-1-RR

    1x10^6 K562 were intracellularly stained with 0.25 ug GAR1 Recombinant antibody (83577-1-RR, Clone:240537C11) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 83577-1-RR

    Affinity and Kinetic Characterization of 83577-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83577-1-RR against Human GAR1 were performed. The affinity constant is below 1 pM.

GAR1 Recombinant antibody

GAR1 {ptg:Brand} Recombinant Antibody for IHC, IF/ICC, ELISA
Cat No. 83577-1-RR
Clone No.240537C11

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, ELISA

Synonyms

snoRNP protein GAR1, NOLA1, 240537C11

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inK562

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:150-1:600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83577-1-RR targets GAR1 in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen GAR1 fusion protein Ag2282 Predict reactive species
Full Name GAR1 ribonucleoprotein homolog (yeast)
Calculated Molecular Weight 217 aa, 22 kDa
GenBank Accession NumberBC003413
Gene Symbol GAR1
Gene ID (NCBI) 54433
RRIDAB_3671188
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9NY12
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GAR1, other named NOLA1, is a subunit of H/ACA and telomerase. Thought is not required for H/ACA protein assembly, GAR1 is necessary for ribosome biogenesis and telomere. H/ACA involves in class specify the sites of uridine-to-pseudouridine. It contains a core domain that flanked by glycine- and arginine-rich(GAR) domains. GAR1 also required for correct processing or intranuclear trafficking of TERC, the RNA component of the telomerase reverse transciptase(TERT) holoenzyme.

Protocols

Product Specific Protocols
IHC protocol for GAR1 antibody 83577-1-RRDownload protocol
IF protocol for GAR1 antibody 83577-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols