CoraLite® Plus 647-conjugated GAPDH (Human Specific) Recombinant antibody

GAPDH (Human Specific) Recombinant Antibody for WB, IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

2G21

Cat no : CL647-80570

Synonyms

GAPDH, GAPD, EC:2.6.99.-, EC:1.2.1.12, CDABP0047

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Validation Data Gallery

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  WB analysis using CL647-80570

  Various lysates were subjected to SDS PAGE followed by western blot with CL647-80570 (GAPDH (Human Specific) antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using CL647-80570

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 647 GAPDH (Human Specific) antibody (CL647-80570, Clone: 2G21 ) at dilution of 1:200.

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  FC experiment of HeLa using CL647-80570

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 647 Anti-Human GAPDH (Human Specific) (CL647-80570, Clone:2G21) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, Jurkat cells
• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL647-80570 targets GAPDH (Human Specific) in WB, IF/ICC, FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: GAPDH (Human Specific) fusion protein Ag0766
• Full Name: glyceraldehyde-3-phosphate dehydrogenase
• Calculated Molecular Weight: 36 kDa
• Observed Molecular Weight: 33 kDa
• GenBank Accession Number: BC004109
• Gene Symbol: GAPDH
• Gene ID (NCBI): 2597
• RRID: AB_2920334
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, binding RNA, RNA transportation, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It is widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocate to the nucleus after the addition of post-translational modifications such as S-nitrosylation. This antibody is raised against full length GAPDH of human origin. It can recognize the 36 kDa GAPDH protein in most cells/tissues. In addition, a band below 36 kDa can always be detected as the isoform or spliced product of GAPDH (PMID: 23885286, 23877755, 19368702). Please note that some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types. For murine tissue samples, conjugated mouse antibody HRP-60004 and rabbit antibody 10494-1-AP are preferable. 80570-1-RR is human specific.

Related Products

Flow Cytometry Perm Buffer (10X)

PF00011-C