GAPDH (Human Specific) Recombinant antibody

GAPDH (Human Specific) Recombinant Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG


Human and More (1)







Cat no : 80570-1-RR



Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, Jurkat cells, K-562 cells
Positive IHC detected inhuman liver cancer tissue, human lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 6 publications below

Product Information

80570-1-RR targets GAPDH (Human Specific) in WB, IHC, IF, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen GAPDH (Human Specific) fusion protein Ag0766
Full Name glyceraldehyde-3-phosphate dehydrogenase
Calculated Molecular Weight 36 kDa
GenBank Accession NumberBC004109
Gene Symbol GAPDH
Gene ID (NCBI) 2597
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, binding RNA, RNA transportation, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It is widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocate to the nucleus after the addition of post-translational modifications such as S-nitrosylation. This antibody is raised against full length GAPDH of human origin. It can recognize the 36 kDa GAPDH protein in most cells/tissues. In addition, a band below 36 kDa can always be detected as the isoform or spliced product of GAPDH (PMID: 23885286, 23877755, 19368702). Please note that some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types. For murine tissue samples, conjugated mouse antibody HRP-60004 and rabbit antibody 10494-1-AP are preferable. 80570-1-RR is human specific.


Product Specific Protocols
WB protocol for GAPDH (Human Specific) antibody 80570-1-RRDownload protocol
IHC protocol for GAPDH (Human Specific) antibody 80570-1-RRDownload protocol
IF protocol for GAPDH (Human Specific) antibody 80570-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Mater Today Bio

Sustained release of naringin from silk-fibroin-nanohydroxyapatite scaffold for the enhancement of bone regeneration.

Authors - Zhi-Hu Zhao

Front Mol Biosci

SLC7A11, a Potential Therapeutic Target Through Induced Ferroptosis in Colon Adenocarcinoma.

Authors - Xin Cheng

J Biochem Mol Toxicol

ATL1 inhibits the proliferation and invasion of trophoblast cells via inhibition of the mTOR signaling pathway

Authors - Guanli Zhang

Diagn Pathol

The multi-omics analyses of acsl1 reveal its translational significance as a tumor microenvironmental and prognostic biomarker in clear cell renal cell carcinoma

Authors - Yang Yang

Medicine (Baltimore)

Integrated analysis of potential biomarkers associated with diabetic periodontitis development based on bioinformatics: An observational study

Authors - Yiran Wu

J Cancer Res Clin Oncol

High expression of cuproptosis-related gene FDX1 in relation to good prognosis and immune cells infiltration in colon adenocarcinoma (COAD)

Authors - Lizong Wang