GAPDH (Human Specific) Recombinant antibody

GAPDH (Human Specific) Recombinant Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

2G21

Cat no : 80570-1-RR

Synonyms

G3PD, GAPD, GAPDH, OK/SW cl.12



Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, Jurkat cells, K-562 cells
Positive IHC detected inhuman liver cancer tissue, human lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 2 publications below

Product Information

80570-1-RR targets GAPDH (Human Specific) in WB, IHC, IF, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen GAPDH (Human Specific) fusion protein Ag0766
Full Name glyceraldehyde-3-phosphate dehydrogenase
Calculated molecular weight 36 kDa
GenBank accession numberBC004109
Gene symbol GAPDH
Gene ID (NCBI) 2597
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, binding RNA, RNA transportation, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It is widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocate to the nucleus after the addition of post-translational modifications such as S-nitrosylation. This antibody is raised against full length GAPDH of human origin. It can recognize the 36 kDa GAPDH protein in most cells/tissues. In addition, a band below 36 kDa can always be detected as the isoform or spliced product of GAPDH (PMID: 23885286, 23877755, 19368702). Please note that some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types. For murine tissue samples, conjugated mouse antibody HRP-60004 and rabbit antibody 10494-1-AP are preferable. 80570-1-RR is human specific.

Protocols

Product Specific Protocols
WB protocol for GAPDH (Human Specific) antibody 80570-1-RRDownload protocol
IHC protocol for GAPDH (Human Specific) antibody 80570-1-RRDownload protocol
IF protocol for GAPDH (Human Specific) antibody 80570-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mater Today Bio

Sustained release of naringin from silk-fibroin-nanohydroxyapatite scaffold for the enhancement of bone regeneration.

Authors - Zhi-Hu Zhao
humanWB

Front Mol Biosci

SLC7A11, a Potential Therapeutic Target Through Induced Ferroptosis in Colon Adenocarcinoma.

Authors - Xin Cheng