GAPDH (Human Specific) Recombinant antibody

GAPDH (Human Specific) Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (2)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

14

CloneNo.

2G21

Cat no : 80570-1-RR

Synonyms

G3PD, GAPD, GAPDH, OK/SW cl.12

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Validation Data Gallery

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  WB analysis using 80570-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 80570-1-RR (GAPDH antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 80570-1-RR

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 80570-1-RR (GAPDH (Human Specific) antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 80570-1-RR

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 80570-1-RR (GAPDH (Human Specific) antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung using 80570-1-RR

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 80570-1-RR (GAPDH (Human Specific) antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung using 80570-1-RR

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 80570-1-RR (GAPDH (Human Specific) antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 80570-1-RR

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using GAPDH (Human Specific) antibody (80570-1-RR, Clone: 2G21 ) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 80570-1-RR

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using GAPDH (Human Specific) antibody (80570-1-RR, Clone: 2G21 ) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells
• Positive IHC detected in: human liver cancer tissue, human lung tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunohistochemistry (IHC): IHC : 1:400-1:1600
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 14 publications below

Product Information

80570-1-RR targets GAPDH (Human Specific) in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: GAPDH (Human Specific) fusion protein Ag0766
• Full Name: glyceraldehyde-3-phosphate dehydrogenase
• Calculated Molecular Weight: 36 kDa
• GenBank Accession Number: BC004109
• Gene Symbol: GAPDH
• Gene ID (NCBI): 2597
• RRID: AB_2918902
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, binding RNA, RNA transportation, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It is widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocate to the nucleus after the addition of post-translational modifications such as S-nitrosylation. This antibody is raised against full length GAPDH of human origin. It can recognize the 36 kDa GAPDH protein in most cells/tissues. In addition, a band below 36 kDa can always be detected as the isoform or spliced product of GAPDH (PMID: 23885286, 23877755, 19368702). Please note that some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types. For murine tissue samples, conjugated mouse antibody HRP-60004 and rabbit antibody 10494-1-AP are preferable. 80570-1-RR is human specific.

Publications

Species	Application	Title
human	WB	Mater Today Bio; Sustained release of naringin from silk-fibroin-nanohydroxyapatite scaffold for the enhancement of bone regeneration.; Authors - Zhi-Hu Zhao
mouse	WB	Free Radic Biol Med; N6-methyladenosine modification of SPOP relieves ferroptosis and diabetic cardiomyopathy by enhancing ubiquitination of VDAC3; Authors - Wei Meng
human	WB	Front Mol Biosci; SLC7A11, a Potential Therapeutic Target Through Induced Ferroptosis in Colon Adenocarcinoma.; Authors - Xin Cheng
human	WB	J Biochem Mol Toxicol; ATL1 inhibits the proliferation and invasion of trophoblast cells via inhibition of the mTOR signaling pathway; Authors - Guanli Zhang
	WB	Diagn Pathol; The multi-omics analyses of acsl1 reveal its translational significance as a tumor microenvironmental and prognostic biomarker in clear cell renal cell carcinoma; Authors - Yang Yang
human	WB	Medicine (Baltimore); Integrated analysis of potential biomarkers associated with diabetic periodontitis development based on bioinformatics: An observational study; Authors - Yiran Wu