GAPDH (Human Specific) Recombinant antibody, PBS Only

GAPDH (Human Specific) Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2G21

Cat no : 80570-1-PBS

Synonyms

G3PD, GAPD, GAPDH, OK/SW cl.12

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Validation Data Gallery

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  WB analysis using 80570-1-RR (same clone as 80570-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 80570-1-RR (GAPDH antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80570-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 80570-1-RR (same clone as 80570-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 80570-1-RR (GAPDH (Human Specific) antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80570-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 80570-1-RR (same clone as 80570-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 80570-1-RR (GAPDH (Human Specific) antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80570-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung using 80570-1-RR (same clone as 80570-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 80570-1-RR (GAPDH (Human Specific) antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80570-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung using 80570-1-RR (same clone as 80570-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 80570-1-RR (GAPDH (Human Specific) antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80570-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 80570-1-RR (same clone as 80570-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using GAPDH (Human Specific) antibody (80570-1-RR, Clone: 2G21 ) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 80570-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 80570-1-RR (same clone as 80570-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using GAPDH (Human Specific) antibody (80570-1-RR, Clone: 2G21 ) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 80570-1-PBS in a different storage buffer formulation.

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Product Information

80570-1-PBS targets GAPDH (Human Specific) in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: GAPDH (Human Specific) fusion protein Ag0766
• Full Name: glyceraldehyde-3-phosphate dehydrogenase
• Calculated Molecular Weight: 36 kDa
• GenBank Accession Number: BC004109
• Gene Symbol: GAPDH
• Gene ID (NCBI): 2597
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, binding RNA, RNA transportation, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It is widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocate to the nucleus after the addition of post-translational modifications such as S-nitrosylation. This antibody is raised against full length GAPDH of human origin. It can recognize the 36 kDa GAPDH protein in most cells/tissues. In addition, a band below 36 kDa can always be detected as the isoform or spliced product of GAPDH (PMID: 23885286, 23877755, 19368702). Please note that some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types. For murine tissue samples, conjugated mouse antibody HRP-60004 and rabbit antibody 10494-1-AP are preferable. 80570-1-RR is human specific.