GADD45A Recombinant antibody, PBS Only (Detector)

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  IHC staining of human stomach cancer using 84376-3-RR (same clone as 84376-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 84376-3-RR (GADD45A antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84376-3-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 84376-3-RR (same clone as 84376-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 84376-3-RR (GADD45A antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84376-3-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 84376-3-RR (same clone as 84376-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using GADD45A antibody (84376-3-RR, Clone: 241597F5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84376-3-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01247-3

  Cytometric bead array standard curve of MP01247-3, GADD45A Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84376-2-PBS. Detection antibody: 84376-3-PBS. Standard: Ag4687. Range: 0.313-40 ng/mL.

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  Affinity and Kinetic Characterization of 84376-3-RR

  Biolayer interferometry (BLl) kinetic assays of 84376-3-RR against Human GADD45A were performed. The affinity constant is below 1 pM.

GADD45A Uni-rAb^TM Recombinant Antibody for IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Cat No. 84376-3-PBS

Clone No.241597F5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Synonyms

GADD45, DNA damage-inducible transcript 1 protein, DDIT-1, DDIT1, DDIT 1

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Product Information

84376-3-PBS targets GADD45A as part of a matched antibody pair:

MP01247-3: 84376-2-PBS capture and 84376-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: GADD45A fusion protein Ag4687
• Full Name: growth arrest and DNA-damage-inducible, alpha
• Calculated Molecular Weight: 165 aa, 18 kDa
• GenBank Accession Number: BC011757
• Gene Symbol: GADD45A
• Gene ID (NCBI): 1647
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P24522
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.