CoraLite®555-conjugated G3BP2 Polyclonal antibody

• Featured Product
• KD/KO Validated

G3BP2 Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite®555 Fluorescent Dye

Cat no : CL555-16276

Synonyms

G3BP 2, G3BP2, KIAA0660

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Validation Data Gallery

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  IF Staining of HeLa using CL555-16276

  Immunofluorescent analysis of (4% PFA) fixed sodium arsenite treated HeLa cells using CoraLite®555 G3BP2 antibody (CL555-16276) at dilution of 1:200.

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  IF Staining of HeLa using CL555-16276

  Immunofluorescent analysis of (4% PFA) fixed sodium arsenite treated HeLa cells using CoraLite®555 G3BP2 antibody (CL555-16276) at dilution of 1:200.

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Tested Applications

• Positive IF/ICC detected in: sodium arsenite treated HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of CL555-16276 is G3BP2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: G3BP2 fusion protein Ag9355
• Full Name: GTPase activating protein (SH3 domain) binding protein 2
• Calculated Molecular Weight: 482aa,54 kDa; 449aa,51 kDa
• Observed Molecular Weight: 65-70 kDa
• GenBank Accession Number: BC011731
• Gene Symbol: G3BP2
• Gene ID (NCBI): 9908
• RRID: AB_2919631
• Conjugate: CoraLite®555 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 557 nm / 570nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage.

Background Information

Stress granules (SGs) are cytoplasmic mRNA-protein condensates formed in response to cellular stressors, such as oxidative stress, ultraviolet radiation, and viral infection (1). The Ras-GTPase-activating protein-binding proteins (G3BPs), consisting of G3BP1 and G3BP2, are key nucleating factors essential for SG formation. They function to protect RNAs from harmful conditions. G3BP2 is mainly distributed in the cytoplasm and participates in the formation of stress granules, cell differentiation, proliferation, and signal transduction. Accumulating evidence has demonstrated that aberrant expression of G3BP2 contributes to cancer initiation and progression, such as high expression of G3BP2 increasing cell stemness, metastasis and chemoresistance in breast cancer.