G3BP2 Polyclonal antibody

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  WB analysis using 16276-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 16276-1-AP (G3BP2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 16276-1-AP

  WB result of G3BP2 antibody (16276-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-G3BP2 transfected HepG2 cells.

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  WB analysis using 16276-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16276-1-AP (G3BP2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis using 16276-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16276-1-AP (G3BP2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis using 16276-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16276-1-AP (G3BP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of T47D cells using 16276-1-AP

  WB result of G3BP2 antibody (16276-1-AP, 1:500) with T47D breast cancer cells.

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  WB analysis of MCF-7 using 16276-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 16276-1-AP (G3BP2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 16276-1-AP

  IP result of anti-G3BP2 (IP:16276-1-AP, 4ug; Detection:16276-1-AP 1:1000) with HeLa cells lysate 2200 ug.

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  IHC staining of human lung cancer using 16276-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16276-1-AP (G3BP2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 16276-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16276-1-AP (G3BP2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 16276-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16276-1-AP (G3BP2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 16276-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16276-1-AP (G3BP2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat kidney using 16276-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 16276-1-AP (G3BP2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat kidney using 16276-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 16276-1-AP (G3BP2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 16276-1-AP

  Immunofluorescent analysis of (4% PFA) fixed sodium arsenite treated HeLa cells using G3BP2 antibody (16276-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of HeLa using 16276-1-AP

  Immunofluorescent analysis of (4% PFA) fixed sodium arsenite treated HeLa cells using G3BP2 antibody (16276-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• Featured Product
• KD/KO Validated

G3BP2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 16276-1-AP

Publications(26)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA

Synonyms

G3BP 2, Ras GTPase-activating protein-binding protein 2, GAP SH3 domain-binding protein 2, GAP SH3 domain binding protein 2, G3BP-2

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Tested Applications

• Positive WB detected in: A549 cells, HEK-293 cells, HepG2 cells, MCF-7 cells, T47D cells, HeLa cells, Jurkat cells, Neuro-2a cells, C2C12 cells, C6 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human lung cancer tissue, mouse liver tissue, rat kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: sodium arsenite treated HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 6 publications below
• WB: See 20 publications below
• IHC: See 3 publications below
• IF: See 11 publications below
• IP: See 5 publications below
• CoIP: See 2 publications below
• RIP: See 1 publications below

Product Information

16276-1-AP targets G3BP2 in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: G3BP2 fusion protein Ag9355
• Full Name: GTPase activating protein (SH3 domain) binding protein 2
• Calculated Molecular Weight: 482aa,54 kDa; 449aa,51 kDa
• Observed Molecular Weight: 65-70 kDa
• GenBank Accession Number: BC011731
• Gene Symbol: G3BP2
• Gene ID (NCBI): 9908
• RRID: AB_2878237
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UN86
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Stress granules (SGs) are cytoplasmic mRNA-protein condensates formed in response to cellular stressors, such as oxidative stress, ultraviolet radiation, and viral infection (1). The Ras-GTPase-activating protein-binding proteins (G3BPs), consisting of G3BP1 and G3BP2, are key nucleating factors essential for SG formation. They function to protect RNAs from harmful conditions. G3BP2 is mainly distributed in the cytoplasm and participates in the formation of stress granules, cell differentiation, proliferation, and signal transduction. Accumulating evidence has demonstrated that aberrant expression of G3BP2 contributes to cancer initiation and progression, such as high expression of G3BP2 increasing cell stemness, metastasis and chemoresistance in breast cancer.

Publications

Species	Application	Title
human	WB,CoIP	Cell; Diverse CMT2 neuropathies are linked to aberrant G3BP interactions in stress granules; Authors - Qinqin Cui
human		Cell; G3BP1 Is a Tunable Switch that Triggers Phase Separation to Assemble Stress Granules.; Authors - Peiguo Yang
human	WB	Cell Rep; hnRNPA2B1 represses the disassembly of arsenite-induced stress granules and is essential for male fertility; Authors - Xiaoli Wang
human	WB,IP	Oncogene; HDAC6-G3BP2 promotes lysosomal-TSC2 and suppresses mTORC1 under ETV4 targeting-induced low-lactate stress in non-small cell lung cancer; Authors - Bei Liu ; KD Validated
human	WB,IF,IP	Oncogene; RIOK1 mediates p53 degradation and radioresistance in colorectal cancer through phosphorylation of G3BP2.; Authors - Yaqi Chen ; KD Validated
human	WB,IHC	Mol Cancer; Invasion-related circular RNA circFNDC3B inhibits bladder cancer progression through the miR-1178-3p/G3BP2/SRC/FAK axis.; Authors - Hongwei Liu ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Karine (Verified Customer) (09-22-2022)

Proteins were extracted from H69 cells using Laemmli lysis buffer (12.5mMNa2HPO4, 15% glycerol, 3% sodium dodecyl sulfate [SDS]). The proteinconcentration was measured with the DC Protein Assay (BIO-RAD) and 30μgof total proteins were loaded onto 12% SDS- polyacrylamide gels forelectrophoresis and transferred onto polyvinylidene difluoride membranes(Millipore). After 1h of blocking with 5% bovine serum albumin preparedin Phosphate-Buffered Saline (PBS)-0.1% Tween-20 buffer, the blots wereincubated overnight at 4°C with the indicated antibody (dilution 1/1000). After 1h of incubation with a horseradish peroxidase-conjugated secondary antibody (1:5,000, Promega), protein bands were visualized using an enhancedchemiluminescence detection kit (Millipore) and and the Syngene Pxi4imaging system (Ozyme).

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: H69 cells

G3BP2 Antibody Western Blot validation (1/1000 dilution) in H69 cells (Cat no:16276-1-AP)