CoraLite® Plus 647-conjugated G3BP1 Monoclonal antibody

• Featured Product
• KD/KO Validated

G3BP1 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig and More (1)

Applications

IF

Conjugate

CoraLite® Plus 647 Fluorescent Dye

Publications

1

CloneNo.

1E4A2

Cat no : CL647-66486

Synonyms

G3BP, G3BP 1, G3BP1, HDH VIII

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Validation Data Gallery

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  IF Staining of HeLa using CL647-66486

  Immunofluorescent analysis of (4% PFA) fixed sodium arsenite treated HeLa cells using CoraLite® Plus 647 G3BP1 antibody (CL647-66486, Clone: 1E4A2 ) at dilution of 1:200, CL488-Phalloidin (green).

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Tested Applications

• Positive IF detected in: sodium arsenite treated HeLa cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

CL647-66486 targets G3BP1 in IF applications and shows reactivity with Human, mouse, rat, pig samples.

• Tested Reactivity: Human, mouse, rat, pig
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: G3BP1 fusion protein Ag3728
• Full Name: GTPase activating protein (SH3 domain) binding protein 1
• Calculated Molecular Weight: 466 aa, 52 kDa
• Observed Molecular Weight: 68 kDa
• GenBank Accession Number: BC006997
• Gene Symbol: G3BP1
• Gene ID (NCBI): 10146
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

GAP SH3 Binding Protein 1 (G3BP1), also named as G3BP, is an effector of stress granule (SG) assembly. SG biology plays an important role in the pathophysiology of TDP-43 in ALS and FTLD-U. G3BP1 can be used as a marker of SG. It has been shown to function downstream of Ras and play a role in RNA metabolism, signal transduction, and proliferation. G3BP1 is a ubiquitously expressed protein that localizes to the cytoplasm in proliferating cells and to the nucleus in non-proliferating cells. G3BP1 has recently been implicated in cancer biology.

Publications

Species	Application	Title
human	IF	Nat Cell Biol; Mammalian IRE1α dynamically and functionally coalesces with stress granules; Authors - Songzi Liu