CoraLite®594-conjugated G3BP1 Monoclonal antibody

G3BP1 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig

Applications

IHC, IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

Publications

2

CloneNo.

1E4A2

Cat no : CL594-66486

Synonyms

G3BP, G3BP 1, G3BP1, HDH VIII

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Validation Data Gallery

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  IF Staining of HeLa using CL594-66486

  Immunofluorescent analysis of (4% PFA) fixed sodium arsenite treated HeLa cells using CoraLite®594 G3BP1 antibody (CL594-66486, Clone: 1E4A2 ) at dilution of 1:200, CL488-Phalloidin (green).

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  IF Staining of HeLa using CL594-66486

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CL594-66486 (G3BP1 antibody) at dilution of 1:100.

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Tested Applications

• Positive IF/ICC detected in: sodium arsenite treated HeLa cells, HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 2 publications below

Product Information

CL594-66486 targets G3BP1 in IHC, IF/ICC applications and shows reactivity with Human, mouse, rat, pig samples.

• Tested Reactivity: Human, mouse, rat, pig
• Cited Reactivity: mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: G3BP1 fusion protein Ag3728
• Full Name: GTPase activating protein (SH3 domain) binding protein 1
• Calculated Molecular Weight: 466 aa, 52 kDa
• Observed Molecular Weight: 68 kDa
• GenBank Accession Number: BC006997
• Gene Symbol: G3BP1
• Gene ID (NCBI): 10146
• RRID: AB_2883591
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

GAP SH3 Binding Protein 1 (G3BP1), also named as G3BP, is an effector of stress granule (SG) assembly. SG biology plays an important role in the pathophysiology of TDP-43 in ALS and FTLD-U. G3BP1 can be used as a marker of SG. It has been shown to function downstream of Ras and play a role in RNA metabolism, signal transduction, and proliferation. G3BP1 is a ubiquitously expressed protein that localizes to the cytoplasm in proliferating cells and to the nucleus in non-proliferating cells. G3BP1 has recently been implicated in cancer biology.

Publications

Species	Application	Title
mouse	IHC	J Mol Cell Cardiol; RBM20S639G mutation is a high genetic risk factor for premature death through RNA-protein condensates.; Authors - Chunyan Wang
	IHC	Nucleic Acids Res; A toolkit for the identification of NEAT1_2/paraspeckle modulators; Authors - Haiyan An