Flotillin 2 Monoclonal antibody, PBS Only

Flotillin 2 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1E11G12

Cat no : 66881-1-PBS

Synonyms

FLOT2, Flotillin-2, ESA1, ESA, Epidermal surface antigen

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Validation Data Gallery

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  WB analysis using 66881-1-Ig (same clone as 66881-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66881-1-Ig (Flotillin 2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 66881-1-PBS in a different storage buffer formulation.

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  WB analysis using 66881-1-Ig (same clone as 66881-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66881-1-Ig (Flotillin 2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66881-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A431 using 66881-1-Ig (same clone as 66881-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 66881-1-Ig (Flotillin 2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66881-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66881-1-Ig (same clone as 66881-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66881-1-Ig (Flotillin 2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66881-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66881-1-Ig (same clone as 66881-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66881-1-Ig (Flotillin 2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66881-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66881-1-Ig (same clone as 66881-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Flotillin 2 antibody (66881-1-Ig, Clone: 1E11G12 ) at dilution of 1:4000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66881-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 66881-1-Ig (same clone as 66881-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.4 ug Flotillin 2 Monoclonal antibody (66881-1-Ig, Clone:1E11G12) and CoraLite488-conjugated Donkey Anti-Mouse IgG(H+L)( SA00013-5)(red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66881-1-PBS in a different storage buffer formulation.

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Product Information

66881-1-PBS targets Flotillin 2 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

• Tested Reactivity: human, mouse, rat, pig, rabbit
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Flotillin 2 fusion protein Ag28308
• Full Name: flotillin 2
• Calculated Molecular Weight: 379 aa, 42 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC017292
• Gene Symbol: Flotillin 2
• Gene ID (NCBI): 2319
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Flotillins are membrane-associated proteins that contain two homologous isoforms, flotillin 1 (FLOT1) and flotillin 2 (FLOT2). They are highly conserved and widely expressed proteins that commonly used as markers of lipid rafts. Flotillins are involved in various signaling pathways, cell adhesion, membrane trafficking and axonal growth.