Flotillin 2 Monoclonal antibody, PBS Only

Flotillin 2 Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig, Rabbit

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1E11G12

Cat no : 66881-1-PBS

Synonyms

ECS 1, ECS1, Epidermal surface antigen, ESA, ESA1, FLOT2, flotillin 2, M17S1

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Validation Data Gallery

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  WB analysis using 66881-1-Ig (same clone as 66881-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66881-1-Ig (Flotillin 2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 66881-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66881-1-Ig (same clone as 66881-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66881-1-Ig (Flotillin 2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66881-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A431 using 66881-1-Ig (same clone as 66881-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 66881-1-Ig (Flotillin 2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66881-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66881-1-Ig (same clone as 66881-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66881-1-Ig (Flotillin 2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66881-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66881-1-Ig (same clone as 66881-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66881-1-Ig (Flotillin 2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66881-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66881-1-Ig (same clone as 66881-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Flotillin 2 antibody (66881-1-Ig, Clone: 1E11G12 ) at dilution of 1:4000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66881-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66881-1-PBS targets Flotillin 2 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat, Pig, Rabbit samples.

• Tested Reactivity: Human, Mouse, Rat, Pig, Rabbit
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Flotillin 2 fusion protein Ag28308
• Full Name: flotillin 2
• Calculated Molecular Weight: 379 aa, 42 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC017292
• Gene Symbol: FLOT2
• Gene ID (NCBI): 2319
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Flotillins are membrane-associated proteins that contain two homologous isoforms, flotillin 1 (FLOT1) and flotillin 2 (FLOT2). They are highly conserved and widely expressed proteins that commonly used as markers of lipid rafts. Flotillins are involved in various signaling pathways, cell adhesion, membrane trafficking and axonal growth.