Flotillin 1 Recombinant antibody, PBS Only

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  WB analysis using 83598-4-RR (same clone as 83598-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83598-4-RR (Flotillin 1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83598-4-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 83598-4-RR (same clone as 83598-4-PBS)

  IP result of anti-Flotillin 1 (IP:83598-4-RR, 4ug; Detection:83598-4-RR 1:3000) with mouse brain tissue lysate 2400 ug. This data was developed using the same antibody clone with 83598-4-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 83598-4-RR (same clone as 83598-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83598-4-RR (Flotillin 1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83598-4-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 83598-4-RR (same clone as 83598-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83598-4-RR (Flotillin 1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83598-4-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 83598-4-RR (same clone as 83598-4-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Flotillin 1 antibody (83598-4-RR, Clone: 240582G6 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83598-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83598-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83598-4-RR against Human Flotillin 1 were performed. The affinity constant is 120 nM.

Flotillin 1 Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 83598-4-PBS

Clone No.240582G6

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

FLOT1, Flotillin-1, 240582G6

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Product Information

83598-4-PBS targets Flotillin 1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Flotillin 1 fusion protein Ag7902
• Full Name: flotillin 1
• Calculated Molecular Weight: 47 kDa
• Observed Molecular Weight: 47 kDa
• GenBank Accession Number: BC001146
• Gene Symbol: Flotillin 1
• Gene ID (NCBI): 10211
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: O75955
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Flotillins are membrane-associated proteins that contain two homologous isoforms, flotillin 1 (FLOT1) and flotillin 2 (FLOT2). They are highly conserved and widely expressed proteins commonly used as lipid raft markers. Flotillins are involved in various signaling pathways, cell adhesion, membrane trafficking and axonal growth.