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Flotillin 1 Recombinant antibody, PBS Only

Flotillin 1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

CloneNo.

240582G6

Cat no : 83598-4-PBS

Synonyms

FLOT1, Flotillin-1, 240582G6

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Flotillin 1 Recombinant antibody (83598-4-RR)

    WB analysis using 83598-4-RR (same clone as 83598-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83598-4-RR (Flotillin 1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83598-4-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using Flotillin 1 Recombinant antibody (83598-4-RR)

    IP experiment of mouse brain using 83598-4-RR (same clone as 83598-4-PBS)

    IP result of anti-Flotillin 1 (IP:83598-4-RR, 4ug; Detection:83598-4-RR 1:3000) with mouse brain tissue lysate 2400 ug. This data was developed using the same antibody clone with 83598-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using Flotillin 1 Recombinant antibody (83598-4-RR)

    IF Staining of Jurkat using 83598-4-RR (same clone as 83598-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using Flotillin 1 antibody (83598-4-RR, Clone: 240582G6 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83598-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83598-4-RR

    Affinity and Kinetic Characterization of 83598-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83598-4-RR against Human Flotillin 1 were performed. The affinity constant is 120 nM.

Product Information

The immunogen of 83598-4-PBS is Flotillin 1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Flotillin 1 fusion protein Ag7902
Full Name flotillin 1
Calculated Molecular Weight 47 kDa
Observed Molecular Weight 47 kDa
GenBank Accession NumberBC001146
Gene Symbol Flotillin 1
Gene ID (NCBI) 10211
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Flotillins are membrane-associated proteins that contain two homologous isoforms, flotillin 1 (FLOT1) and flotillin 2 (FLOT2). They are highly conserved and widely expressed proteins commonly used as lipid raft markers. Flotillins are involved in various signaling pathways, cell adhesion, membrane trafficking and axonal growth.