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Flotillin 1 Recombinant antibody

Flotillin 1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

CloneNo.

240582G6

Cat no : 83598-4-RR

Synonyms

FLOT1, Flotillin-1, 240582G6

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Flotillin 1 Recombinant antibody (83598-4-RR)

    WB analysis using 83598-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83598-4-RR (Flotillin 1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using Flotillin 1 Recombinant antibody (83598-4-RR)

    IP experiment of mouse brain using 83598-4-RR

    IP result of anti-Flotillin 1 (IP:83598-4-RR, 4ug; Detection:83598-4-RR 1:3000) with mouse brain tissue lysate 2400 ug.

  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using Flotillin 1 Recombinant antibody (83598-4-RR)

    IF Staining of Jurkat using 83598-4-RR

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using Flotillin 1 antibody (83598-4-RR, Clone: 240582G6 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 83598-4-RR

    Affinity and Kinetic Characterization of 83598-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83598-4-RR against Human Flotillin 1 were performed. The affinity constant is 120 nM.

Tested Applications

Positive WB detected inU-87 MG cells, LNCaP cells, Raji cells, mouse heart tissue, mouse brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IF/ICC detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83598-4-RR is Flotillin 1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Flotillin 1 fusion protein Ag7902
Full Name flotillin 1
Calculated Molecular Weight 47 kDa
Observed Molecular Weight 47 kDa
GenBank Accession NumberBC001146
Gene Symbol Flotillin 1
Gene ID (NCBI) 10211
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Flotillins are membrane-associated proteins that contain two homologous isoforms, flotillin 1 (FLOT1) and flotillin 2 (FLOT2). They are highly conserved and widely expressed proteins commonly used as lipid raft markers. Flotillins are involved in various signaling pathways, cell adhesion, membrane trafficking and axonal growth.

Protocols

Product Specific Protocols
WB protocol for Flotillin 1 antibody 83598-4-RRDownload protocol
IF protocol for Flotillin 1 antibody 83598-4-RRDownload protocol
IP protocol for Flotillin 1 antibody 83598-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols