Flotillin 1 Recombinant antibody

Flotillin 1 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

CloneNo.

240582G6

Cat no : 83598-4-RR

Synonyms

FLOT1, Flotillin-1, 240582G6

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Validation Data Gallery

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  WB analysis using 83598-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83598-4-RR (Flotillin 1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 83598-4-RR

  IP result of anti-Flotillin 1 (IP:83598-4-RR, 4ug; Detection:83598-4-RR 1:3000) with mouse brain tissue lysate 2400 ug.

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  IF Staining of Jurkat using 83598-4-RR

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using Flotillin 1 antibody (83598-4-RR, Clone: 240582G6 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 83598-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83598-4-RR against Human Flotillin 1 were performed. The affinity constant is 120 nM.

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Tested Applications

• Positive WB detected in: U-87 MG cells, LNCaP cells, Raji cells, mouse heart tissue, mouse brain tissue, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IF/ICC detected in: Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83598-4-RR is Flotillin 1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Flotillin 1 fusion protein Ag7902
• Full Name: flotillin 1
• Calculated Molecular Weight: 47 kDa
• Observed Molecular Weight: 47 kDa
• GenBank Accession Number: BC001146
• Gene Symbol: Flotillin 1
• Gene ID (NCBI): 10211
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Flotillins are membrane-associated proteins that contain two homologous isoforms, flotillin 1 (FLOT1) and flotillin 2 (FLOT2). They are highly conserved and widely expressed proteins commonly used as lipid raft markers. Flotillins are involved in various signaling pathways, cell adhesion, membrane trafficking and axonal growth.