Fibrinogen Gamma Chain Monoclonal antibody, PBS Only

Fibrinogen Gamma Chain Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgM

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2B2B8

Cat no : 66158-1-PBS

Synonyms

FGG, PRO2061, 2B2B8

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Validation Data Gallery

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  WB analysis of L02 using 66158-1-Ig (same clone as 66158-1-PBS)

  L02 cells were subjected to SDS PAGE followed by western blot with 66158-1-Ig (Fibrinogen gamma chain antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66158-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human peripheral blood leukocyte using 66158-1-Ig (same clone as 66158-1-PBS)

  human peripheral blood leukocyte were subjected to SDS PAGE followed by western blot with 66158-1-Ig (Fibrinogen Gamma Chain antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66158-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human peripheral blood platelets using 66158-1-Ig (same clone as 66158-1-PBS)

  human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 66158-1-Ig (Fibrinogen Gamma Chain antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66158-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66158-1-Ig (same clone as 66158-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66158-1-Ig (Fibrinogen Gamma Chain antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66158-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66158-1-Ig (same clone as 66158-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66158-1-Ig (Fibrinogen Gamma Chain antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66158-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 66158-1-Ig (same clone as 66158-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66158-1-Ig (Fibrinogen gamma chain antibody at dilution of 1:50. This data was developed using the same antibody clone with 66158-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 66158-1-Ig (same clone as 66158-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66158-1-Ig (Fibrinogen gamma chain antibody at dilution of 1:50. This data was developed using the same antibody clone with 66158-1-PBS in a different storage buffer formulation.

• 

  IF Staining of L02 using 66158-1-Ig (same clone as 66158-1-PBS)

  Immunofluorescent analysis of L02 cells using 66158-1-Ig (Fibrinogen gamma chain antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L). This data was developed using the same antibody clone with 66158-1-PBS in a different storage buffer formulation.

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Product Information

66158-1-PBS targets Fibrinogen Gamma Chain in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgM
• Class: Monoclonal
• Type: Antibody
• Immunogen: Fibrinogen Gamma Chain fusion protein Ag8817
• Full Name: fibrinogen gamma chain
• Calculated Molecular Weight: 453aa,52 kDa; 437aa,50 kDa
• Observed Molecular Weight: 52 kDa
• GenBank Accession Number: BC007044
• Gene Symbol: Fibrinogen Gamma Chain
• Gene ID (NCBI): 2266
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Fibrinogen is a soluble plasma glycoprotein synthesized in the liver. It is composed of two sets of three structurally different subunits: alpha (FGA), beta (FGB), gamma (FGG). Fibrinogen is converted by thrombin into fibrin during blood coagulation. Fibrinogen and fibrin play overlapping roles in blood clotting, fibrinolysis, cellular and matrix interactions, the inflammatory response, wound healing, and neoplasia (PMID: 16102057). FGG is the gamma chain of fibrinogen. Mutations in the gene of FGG lead to several disorders, including dysfibrinogenemia, hypofibrinogenemia and thrombophilia.