Fibrinogen Gamma Chain Monoclonal antibody
Fibrinogen Gamma Chain Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Mouse / IgM
Reactivity
human
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
2B2B8
Cat no : 66158-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | human peripheral blood leukocyte, L02 cells, human peripheral blood platelets |
| Positive IHC detected in | human liver cancer tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | L02 cells |
Mouse monoclonal antibodies of IgM isotype can be detected with "anti-mouse IgG (H+L)" secondary antibodies.
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| IHC | See 1 publications below |
Product Information
66158-1-Ig targets Fibrinogen Gamma Chain in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgM |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Fibrinogen Gamma Chain fusion protein Ag8817 |
| Full Name | fibrinogen gamma chain |
| Calculated Molecular Weight | 453aa,52 kDa; 437aa,50 kDa |
| Observed Molecular Weight | 52 kDa |
| GenBank Accession Number | BC007044 |
| Gene Symbol | Fibrinogen Gamma Chain |
| Gene ID (NCBI) | 2266 |
| RRID | AB_2881554 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Fibrinogen is a soluble plasma glycoprotein synthesized in the liver. It is composed of two sets of three structurally different subunits: alpha (FGA), beta (FGB), gamma (FGG). Fibrinogen is converted by thrombin into fibrin during blood coagulation. Fibrinogen and fibrin play overlapping roles in blood clotting, fibrinolysis, cellular and matrix interactions, the inflammatory response, wound healing, and neoplasia (PMID: 16102057). FGG is the gamma chain of fibrinogen. Mutations in the gene of FGG lead to several disorders, including dysfibrinogenemia, hypofibrinogenemia and thrombophilia.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Fibrinogen Gamma Chain antibody 66158-1-Ig | Download protocol |
| IHC protocol for Fibrinogen Gamma Chain antibody 66158-1-Ig | Download protocol |
| IF protocol for Fibrinogen Gamma Chain antibody 66158-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Front Genet Elevated Serum FGG Levels Prognosticate and Promote the Disease Progression in Prostate Cancer. | ||
Mol Ther Nucleic Acids Antithrombotic Effect of shRNA Target F12 Mediated by Adeno-Associated Virus. |
