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Ferroptosis Essentials Antibody Kit

Host

Rabbit/Mouse

Reactivity

Target dependent

Applications

Target dependent

Conjugate

Unconjugated

Cat no : PK30002

Validation Data Gallery

Applications:
  • Various lysates were subjected to SDS PAGE followed by western blot with 67763-1-Ig (GPX4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

    Various lysates were subjected to SDS PAGE followed by western blot with 67763-1-Ig (GPX4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Various lysates were subjected to SDS PAGE followed by western blot with 26864-1-AP (SLC7A11/xCT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

    Various lysates were subjected to SDS PAGE followed by western blot with 26864-1-AP (SLC7A11/xCT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB result of KEAP1 antibody (80744-1-RR; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KEAP1 transfected HEK-293 cells.

    WB result of KEAP1 antibody (80744-1-RR; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KEAP1 transfected HEK-293 cells.

  • Non-treated and MG 132 treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 80593-1-RR (NRF2, NFE2L2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

    Non-treated and MG 132 treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 80593-1-RR (NRF2, NFE2L2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • Various lysates were subjected to SDS PAGE followed by western blot with 22401-1-AP (ACSL4 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

    Various lysates were subjected to SDS PAGE followed by western blot with 22401-1-AP (ACSL4 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IP result of anti-NRF2, NFE2L2(IP:80593-1-RR, 4ug; Detection:80593-1-RR 1:800) with HeLa cells lysate 2520 ug.

    IP result of anti-NRF2, NFE2L2(IP:80593-1-RR, 4ug; Detection:80593-1-RR 1:800) with HeLa cells lysate 2520 ug.

  • IP Result of anti-ACSL4/FACL4 (IP:22401-1-AP, 4ug; Detection:22401-1-AP 1:1000) with COLO 320 cells lysate 2000ug.

    IP Result of anti-ACSL4/FACL4 (IP:22401-1-AP, 4ug; Detection:22401-1-AP 1:1000) with COLO 320 cells lysate 2000ug.

  • Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67763-1-Ig (GPX4 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67763-1-Ig (GPX4 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 26864-1-AP (SLC7A11/xCT antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 26864-1-AP (SLC7A11/xCT antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 80744-1-RR (KEAP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 80744-1-RR (KEAP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemical analysis of paraffin-embedded human placenta slide using 22401-1-AP (ACSL4 antibody at dilution of 1:50.

    Immunohistochemical analysis of paraffin-embedded human placenta slide using 22401-1-AP (ACSL4 antibody at dilution of 1:50.

  • Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GPX4 antibody (67763-1-Ig, Clone: 3F5G5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GPX4 antibody (67763-1-Ig, Clone: 3F5G5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using KEAP1 antibody (80744-1-RR, Clone: 5O18 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using KEAP1 antibody (80744-1-RR, Clone: 5O18 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescent analysis of (-20°C Ethanol) fixed MG132 treated HepG2 cells using NRF2, NFE2L2 antibody (80593-1-RR, Clone: 1I21 ) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

    Immunofluorescent analysis of (-20°C Ethanol) fixed MG132 treated HepG2 cells using NRF2, NFE2L2 antibody (80593-1-RR, Clone: 1I21 ) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

Product Information

The Ferroptosis Essentials Antibody Kit provides a cost-effective tool for studying key proteins involved in the ferroptosis pathway. Perfect for researchers starting a new project, screening multiple prospective targets or those who simply require less volume.


Kit Components

The Ferroptosis Essentials Antibody Kit contains antibodies against 5 key protein targets playing critical roles in the ferroptotic cell death pathway. 


AntigenCatalog No.Host, clonalityTested ReactivityApplicationsVolume
GPX467763-1-Ig            Mouse monoclonalH, M, R, Rb, Pg, Dg, Hm, C, ZWB, IHC, IF, ELISA20 uL
SLC7A11/xCT26864-1-AP            Rabbit polyclonalHWB, IP, IHC, ELISA20 uL
KEAP180744-1-RR            Rabbit monoclonalH, M, RWB, IHC, IF, ELISA20 uL
NRF280593-1-RR            Rabbit monoclonalH, MWB, IHC, IF/ICC, FC (Intra), IP, ELISA20 uL
ACSL422401-1-AP            Rabbit polyclonalH, MWB, IP, IHC, ELISA20 uL


Also see our 'Ferroptosis Expanded Antibody Kit' on the following page https://www.ptglab.com/products/Ferroptosis-Expanded-Antibody-Kit-PK30003.htm

Storage

Store at -20°C. Stable for one year from the date of receipt.

Background Information

Ferroptosis is an iron-dependent form of regulated cell death characterized by an increase in intracellular reactive oxygen species (ROS) levels. The peroxidase GPX4, whose activity relies on glutathione (GSH) biosynthesis, is a key regulator of the ferroptosis pathway. GPX4 utilizes GSH as a cofactor to reduce intracellular lipid peroxides. Inactivation of GPX4 caused by intracellular GSH depletion leads to ROS accumulation, thereby triggering ferroptosis. Ferroptosis can also be regulated by the cell surface cysteine-glutamate antiporter (system xc-) consisting of SLC7A11 and SLC3A2 in conjunction with the glutathione metabolic pathway. Inhibition of system xc- prevents glutathione synthesis by inhibiting cysteine absorption, leading to oxidative stress and impairment of GPX4 activity, which in turn promotes ferroptosis. The KEAP1-NRF2 pathway has been shown to play a protective role against ferroptosis in multiple disease models. ACSL4, an enzyme mediating fatty acid metabolism acts as a key driver and biomarker of ferroptosis under specific conditions.


Standard Protocols

Click here to view our standard protocols for various applications including WB, IP, IHC, IF, FC, and ELISA.

Cited in Article as

PK30002, Ferroptosis Essentials Antibody Kit, Proteintech, IL, USA