SLC7A11/xCT Polyclonal antibody

• Featured Product
• KD/KO Validated

SLC7A11/xCT Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF, IP, CoIP, RIP, ELISA

Conjugate

Unconjugated

Publications

215

Reviews (4)

Cat no : 26864-1-AP

Synonyms

xCT, SLC7A11, Cystine/glutamate transporter, CCBR1, Amino acid transport system xc-

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Validation Data Gallery

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  WB analysis using 26864-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26864-1-AP (SLC7A11/xCT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of U2OS using 26864-1-AP

  U2OS cells were subjected to SDS PAGE followed by western blot with 26864-1-AP (SLC7A11/xCT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of A549 using 26864-1-AP

  IP result of anti-SLC7A11/xCT (IP:26864-1-AP, 4ug; Detection:26864-1-AP 1:800) with A549 cells lysate 960 ug.

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  IP experiment of HepG2 using 26864-1-AP

  IP result of anti-SLC7A11/xCT (IP:26864-1-AP, 4ug; Detection:26864-1-AP 1:800) with HepG2 cells lysate 1720 ug.

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  IHC staining of human renal cell carcinoma using 26864-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 26864-1-AP (SLC7A11/xCT antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human renal cell carcinoma using 26864-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 26864-1-AP (SLC7A11/xCT antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: A549 cells, U2OS cells, BxPC-3 cells, HepG2 cells
• Positive IP detected in: A549 cells, HepG2 cells
• Positive IHC detected in: human renal cell carcinoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

For optimal WB detection with 26864-1-AP, we do not recommend boiling the sample after lysis.

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 8 publications below
• WB: See 189 publications below
• IHC: See 43 publications below
• IF: See 13 publications below
• IP: See 1 publications below
• CoIP: See 2 publications below
• RIP: See 1 publications below

Product Information

26864-1-AP targets SLC7A11/xCT in WB, IHC, IF, IP, CoIP, RIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, chicken, goat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SLC7A11/xCT fusion protein Ag25431
• Full Name: solute carrier family 7, (cationic amino acid transporter, y+ system) member 11
• Calculated Molecular Weight: 55 kDa
• Observed Molecular Weight: 55 kDa, 35-40 kDa
• GenBank Accession Number: BC012087
• Gene Symbol: SLC7A11
• Gene ID (NCBI): 23657
• RRID: AB_2880661
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SLC7A11 (also known as xCT) is a membrane transporter involved in the uptake of cystine. It promotes glutathione synthesis through the uptake of cystine and the concomitant release of glutamate. This, in turn, protects cells from oxidative stress, maintains cellular redox balance, and prevents cell death induced by lipid peroxidation. SLC7A11 has been identified as a potential target for cancer therapeutics. It is overexpressed in multiple malignant tumors and is closely associated with the growth, prognosis, metastasis, and treatment response of various cancers including lung cancer. 26864-1-AP recognizes the 55 kDa and 35-40 kDa protein similar to the paper published (PMID: 32188872, 19156441).

Publications

Species	Application	Title
human	WB	Cell Res; Mitochondria-localized cGAS suppresses ferroptosis to promote cancer progression; Authors - Shiqiao Qiu
human	WB	Drug Resist Updat; Upregulation of CoQ shifts ferroptosis dependence from GPX4 to FSP1 in acquired radioresistance; Authors - Xu Lin
human	WB	Nat Immunol; Glutathione peroxidase 4-regulated neutrophil ferroptosis induces systemic autoimmunity.; Authors - Pengchong Li
human	WB	ACS Nano; Engineering MMP-2 Activated Nanoparticles Carrying B7-H3 Bispecific Antibodies for Ferroptosis-Enhanced Glioblastoma Immunotherapy; Authors - Rangrang Fan
human	WB	Nat Commun; URI alleviates tyrosine kinase inhibitors-induced ferroptosis by reprogramming lipid metabolism in p53 wild-type liver cancers; Authors - Zhiwen Ding
human	WB,IHC	J Clin Invest; PRMT5 mediated FUBP1 methylation accelerates prostate cancer progression; Authors - Weiwei Yan

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Chloe (Verified Customer) (11-01-2023)

Great antibody works pretty well for a western blot with 1:1000 dilution.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Breast Cancer Cell Lines

SLC7A11/xCT Antibody Western Blot validation (1:1000 dilution) in Breast Cancer Cell Lines (Cat no:26864-1-AP)

FH

James (Verified Customer) (11-18-2022)

Detection of SLC7A11 in liver protein extracts

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: Liver

SLC7A11/xCT Antibody Western Blot validation (1/1000 dilution) in Liver (Cat no:26864-1-AP)

FH

Melanie (Verified Customer) (05-11-2022)

Tested the antibody in Hek293T cells. Had to dilute the antibody 1:50000, since the signal was to strong. Very clear gang at the calculated MW. Good antibody!

• Applications: Western Blot
• Cell Tissue Type: Hek293T

FH

Amélie (Verified Customer) (05-03-2022)

We used the AB on Bone Marrow derived Macrophages, where Slc7a11 is KO (validate by RT PCR), in Western blot. We did not found a specific staining in the right size.

• Applications: Western Blot
• Primary Antibody Dilution: 1000
• Cell Tissue Type: Bone Marrow derived macrophages from WT or KO mice

SLC7A11/xCT Antibody Western Blot validation (1000 dilution) in Bone Marrow derived macrophages from WT or KO mice (Cat no:26864-1-AP)