ACSL4/FACL4 Polyclonal antibody

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  WB analysis using 22401-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22401-1-AP (ACSL4/FACL4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 22401-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22401-1-AP (ACSL4/FACL4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 22401-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22401-1-AP (ACSL4/FACL4 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 22401-1-AP

  WB result of ACSL4/FACL4 antibody (22401-1-AP; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ACSL4/FACL4 transfected HeLa cells.

• 

  WB analysis using 22401-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22401-1-AP (ACSL4/FACL4 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 22401-1-AP

  IP result of anti-ACSL4/FACL4 (IP:22401-1-AP, 4ug; Detection:22401-1-AP 1:5000) with HEK-293 cells lysate 1400 ug.

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  IHC staining of mouse brain using 22401-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 22401-1-AP (ACSL4/FACL4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

ACSL4/FACL4 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 22401-1-AP

Publications(231)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF, IP, CoIP, ELISA

Synonyms

ACSL4, FACL4, ACS4, ACSL 4, Arachidonate--CoA ligase

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Tested Applications

• Positive WB detected in: BV-2 cells, HEK-293 cells, HeLa cells, HepG2 cells, COLO 320 cells, NIH/3T3 cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 8 publications below
• WB: See 205 publications below
• IHC: See 41 publications below
• IF: See 23 publications below
• IP: See 4 publications below
• CoIP: See 3 publications below

Product Information

22401-1-AP targets ACSL4/FACL4 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, duck
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ACSL4/FACL4 fusion protein Ag18085
• Full Name: acyl-CoA synthetase long-chain family member 4
• Calculated Molecular Weight: 711 aa, 79 kDa
• Observed Molecular Weight: 79 kDa, 74 kDa
• GenBank Accession Number: BC034959
• Gene Symbol: ACSL4
• Gene ID (NCBI): 2182
• RRID: AB_2832995
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O60488
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Acyl-CoA synthetase long-chain family member 4 (Acsl4), an important enzyme involved in lipid metabolism, participates in ferroptosis by converting free AA into arachidonoyl-CoA to generate lipid hydroperoxides. Recent studies revealed that ACSL4 is involved in biological responses including inflammation, steroidogenesis, cell death, female fertility, and cancer. Acsl4 has two isoforms: 79 kDa and 75 kDa. ACSL4 also can be detected in 70 kDa(PMID:35326233).

Publications

Species	Application	Title
human	WB	Nature; 7-Dehydrocholesterol dictates ferroptosis sensitivity; Authors - Yaxu Li
human	CoIP	Cell Res; Mitochondria-localized cGAS suppresses ferroptosis to promote cancer progression; Authors - Shiqiao Qiu
human	WB	Nat Commun; URI alleviates tyrosine kinase inhibitors-induced ferroptosis by reprogramming lipid metabolism in p53 wild-type liver cancers; Authors - Zhiwen Ding
human	WB	Adv Sci (Weinh); Development and Application of the CRISPR-dcas13d-eIF4G Translational Regulatory System to Inhibit Ferroptosis in Calcium Oxalate Crystal-Induced Kidney Injury; Authors - Ziqi He
human	WB	Adv Sci (Weinh); Sirtuin1 Suppresses Calcium Oxalate Nephropathy via Inhibition of Renal Proximal Tubular Cell Ferroptosis Through PGC-1α-mediated Transcriptional Coactivation; Authors - Chen Duan
human	WB	Mol Ther; STING/ACSL4 axis-dependent ferroptosis and inflammation promote hypertension-associated chronic kidney disease; Authors - Li Gao