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ACSL4/FACL4 Polyclonal antibody

ACSL4/FACL4 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, Mouse, rat and More (2)

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 22401-1-AP

Synonyms

ACS4, ACSL4, ACSL4/FACL4, FACL4, LACS 4, LACS4, MRX63, MRX68



Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells, NIH/3T3 cells, COLO 320 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22401-1-AP targets ACSL4/FACL4 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, Mouse, rat samples.

Tested Reactivity human, Mouse, rat
Cited Reactivityhuman, mouse, rat, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ACSL4/FACL4 fusion protein Ag18085
Full Name acyl-CoA synthetase long-chain family member 4
Calculated Molecular Weight 711 aa, 79 kDa
Observed Molecular Weight 79 kDa, 74 kDa
GenBank Accession NumberBC034959
Gene Symbol ACSL4
Gene ID (NCBI) 2182
RRIDAB_2832995
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Acyl-CoA synthetase long-chain family member 4 (Acsl4), an important enzyme involved in lipid metabolism, participates in ferroptosis by converting free AA into arachidonoyl-CoA to generate lipid hydroperoxides. Recent studies revealed that ACSL4 is involved in biological responses including inflammation, steroidogenesis, cell death, female fertility, and cancer. Acsl4 has two isoforms: 79 kDa and 75 kDa. ACSL4 also can be detected in 70 kDa(PMID:35326233).

Protocols

Product Specific Protocols
WB protocol for ACSL4/FACL4 antibody 22401-1-APDownload protocol
IHC protocol for ACSL4/FACL4 antibody 22401-1-APDownload protocol
IP protocol for ACSL4/FACL4 antibody 22401-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanCoIP

Cell Res

Mitochondria-localized cGAS suppresses ferroptosis to promote cancer progression

Authors - Shiqiao Qiu
humanWB

Nat Commun

URI alleviates tyrosine kinase inhibitors-induced ferroptosis by reprogramming lipid metabolism in p53 wild-type liver cancers

Authors - Zhiwen Ding
humanWB

Mol Ther

STING/ACSL4 axis-dependent ferroptosis and inflammation promote hypertension-associated chronic kidney disease

Authors - Li Gao
humanWB

EMBO J

FUNDC1 regulates mitochondrial dynamics at the ER-mitochondrial contact site under hypoxic conditions.

Authors - Wenxian Wu
humanWB

Redox Biol

Non-enzymatic lipid peroxidation initiated by photodynamic therapy drives a distinct ferroptosis-like cell death pathway.

Authors - Sufang Shui
humanWB,IF

Redox Biol

Glia maturation factor-β induces ferroptosis by impairing chaperone-mediated autophagic degradation of ACSL4 in early diabetic retinopathy.

Authors - Caiying Liu