• Featured Product
  • KD/KO Validated

ACSL4/FACL4 Polyclonal antibody

ACSL4/FACL4 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, Mouse, rat and More (2)

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 22401-1-AP

Synonyms

ACS4, ACSL4, ACSL4/FACL4, FACL4, LACS 4, LACS4, MRX63, MRX68



Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells, NIH/3T3 cells, COLO 320 cells
Positive IP detected inCOLO 320 cells
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

22401-1-AP targets ACSL4/FACL4 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, Mouse, rat samples.

Tested Reactivity human, Mouse, rat
Cited Reactivityhuman, mouse, rat, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ACSL4/FACL4 fusion protein Ag18085
Full Name acyl-CoA synthetase long-chain family member 4
Calculated molecular weight 711 aa, 79 kDa
Observed molecular weight 79 kDa, 74 kDa
GenBank accession numberBC034959
Gene symbol ACSL4
Gene ID (NCBI) 2182
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Acyl-CoA synthetase long-chain family member 4 (Acsl4), an important enzyme involved in lipid metabolism, participates in ferroptosis by converting free AA into arachidonoyl-CoA to generate lipid hydroperoxides. Recent studies revealed that ACSL4 is involved in biological responses including inflammation, steroidogenesis, cell death, female fertility, and cancer. Acsl4 has two isoforms: 79 kDa and 75 kDa. ACSL4 also can be detected in 70 kDa(PMID:35326233).

Protocols

Product Specific Protocols
WB protocol for ACSL4/FACL4 antibody 22401-1-APDownload protocol
IHC protocol for ACSL4/FACL4 antibody 22401-1-APDownload protocol
IP protocol for ACSL4/FACL4 antibody 22401-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanCoIP

Cell Res

Mitochondria-localized cGAS suppresses ferroptosis to promote cancer progression

Authors - Shiqiao Qiu
humanWB

Mol Ther

STING/ACSL4 axis-dependent ferroptosis and inflammation promote hypertension-associated chronic kidney disease

Authors - Li Gao
humanWB

EMBO J

FUNDC1 regulates mitochondrial dynamics at the ER-mitochondrial contact site under hypoxic conditions.

Authors - Wenxian Wu
humanWB

Redox Biol

Non-enzymatic lipid peroxidation initiated by photodynamic therapy drives a distinct ferroptosis-like cell death pathway.

Authors - Sufang Shui
humanWB,IF

Redox Biol

Glia maturation factor-β induces ferroptosis by impairing chaperone-mediated autophagic degradation of ACSL4 in early diabetic retinopathy.

Authors - Caiying Liu

EMBO J

Reduced ER-mitochondria connectivity promotes neuroblastoma multidrug resistance.

Authors - Jorida Çoku