ACSL4/FACL4 Polyclonal antibody

• Featured Product
• KD/KO Validated

ACSL4/FACL4 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, Mouse, rat and More (2)

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Publications

70

Cat no : 22401-1-AP

Synonyms

ACS4, ACSL4, ACSL4/FACL4, FACL4, LACS 4, LACS4, MRX63, MRX68

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Validation Data Gallery

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  WB analysis using 22401-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22401-1-AP (ACSL4/FACL4 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 22401-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22401-1-AP (ACSL4/FACL4 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 22401-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22401-1-AP (ACSL4/FACL4 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 22401-1-AP

  WB result of ACSL4/FACL4 antibody (22401-1-AP; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ACSL4/FACL4 transfected HeLa cells.

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  IP experiment of COLO 320 using 22401-1-AP

  IP Result of anti-ACSL4/FACL4 (IP:22401-1-AP, 4ug; Detection:22401-1-AP 1:1000) with COLO 320 cells lysate 2000ug.

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  IHC staining of human placenta using 22401-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta slide using 22401-1-AP (ACSL4/FACL4 antibody at dilution of 1:50.

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  IHC staining of human placenta using 22401-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta slide using 22401-1-AP (ACSL4/FACL4 antibody at dilution of 1:50.

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, HepG2 cells, NIH/3T3 cells, COLO 320 cells
• Positive IP detected in: COLO 320 cells
• Positive IHC detected in: human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
• Immunohistochemistry (IHC): IHC : 1:20-1:200

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 4 publications below
• WB: See 60 publications below
• IHC: See 14 publications below
• IF: See 3 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Product Information

22401-1-AP targets ACSL4/FACL4 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, Mouse, rat samples.

• Tested Reactivity: human, Mouse, rat
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ACSL4/FACL4 fusion protein Ag18085
• Full Name: acyl-CoA synthetase long-chain family member 4
• Calculated molecular weight: 711 aa, 79 kDa
• Observed molecular weight: 79 kDa, 74 kDa
• GenBank accession number: BC034959
• Gene symbol: ACSL4
• Gene ID (NCBI): 2182
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Acyl-CoA synthetase long-chain family member 4 (Acsl4), an important enzyme involved in lipid metabolism, participates in ferroptosis by converting free AA into arachidonoyl-CoA to generate lipid hydroperoxides. Recent studies revealed that ACSL4 is involved in biological responses including inflammation, steroidogenesis, cell death, female fertility, and cancer. Acsl4 has two isoforms: 79 kDa and 75 kDa. ACSL4 also can be detected in 70 kDa(PMID:35326233).

Publications

Species	Application	Title
human	CoIP	Cell Res; Mitochondria-localized cGAS suppresses ferroptosis to promote cancer progression; Authors - Shiqiao Qiu
human	WB	Mol Ther; STING/ACSL4 axis-dependent ferroptosis and inflammation promote hypertension-associated chronic kidney disease; Authors - Li Gao
human	WB	EMBO J; FUNDC1 regulates mitochondrial dynamics at the ER-mitochondrial contact site under hypoxic conditions.; Authors - Wenxian Wu
human	WB	Redox Biol; Non-enzymatic lipid peroxidation initiated by photodynamic therapy drives a distinct ferroptosis-like cell death pathway.; Authors - Sufang Shui
human	WB,IF	Redox Biol; Glia maturation factor-β induces ferroptosis by impairing chaperone-mediated autophagic degradation of ACSL4 in early diabetic retinopathy.; Authors - Caiying Liu
		EMBO J; Reduced ER-mitochondria connectivity promotes neuroblastoma multidrug resistance.; Authors - Jorida Çoku