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- KD/KO Validated
ACSL4/FACL4 Polyclonal antibody
ACSL4/FACL4 Polyclonal Antibody for IHC, IP, WB, ELISA
Cat no : 22401-1-AP
|Positive WB detected in||HEK-293 cells, HeLa cells, HepG2 cells, NIH/3T3 cells, COLO 320 cells|
|Positive IP detected in||COLO 320 cells|
|Positive IHC detected in||human placenta tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:2000-1:12000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
22401-1-AP targets ACSL4/FACL4 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, Mouse, rat samples.
|Tested Reactivity||human, Mouse, rat|
|Cited Reactivity||human, mouse, rat, pig|
|Host / Isotype||Rabbit / IgG|
|Immunogen||ACSL4/FACL4 fusion protein Ag18085|
|Full Name||acyl-CoA synthetase long-chain family member 4|
|Calculated molecular weight||711 aa, 79 kDa|
|Observed molecular weight||79 kDa, 74 kDa|
|GenBank accession number||BC034959|
|Gene ID (NCBI)||2182|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Acyl-CoA synthetase long-chain family member 4 (Acsl4), an important enzyme involved in lipid metabolism, participates in ferroptosis by converting free AA into arachidonoyl-CoA to generate lipid hydroperoxides. Recent studies revealed that ACSL4 is involved in biological responses including inflammation, steroidogenesis, cell death, female fertility, and cancer. Acsl4 has two isoforms: 79 kDa and 75 kDa. ACSL4 also can be detected in 70 kDa(PMID:35326233).
Mitochondria-localized cGAS suppresses ferroptosis to promote cancer progression
STING/ACSL4 axis-dependent ferroptosis and inflammation promote hypertension-associated chronic kidney disease
FUNDC1 regulates mitochondrial dynamics at the ER-mitochondrial contact site under hypoxic conditions.
Non-enzymatic lipid peroxidation initiated by photodynamic therapy drives a distinct ferroptosis-like cell death pathway.
Glia maturation factor-β induces ferroptosis by impairing chaperone-mediated autophagic degradation of ACSL4 in early diabetic retinopathy.
Reduced ER-mitochondria connectivity promotes neuroblastoma multidrug resistance.