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CoraLite® Plus 647-conjugated Fascin Monoclonal antibody

Fascin Monoclonal Antibody for IF, FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IF, FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

1B5G3

Cat no : CL647-66321

Synonyms

55 kDa actin bundling protein, FAN1, Fascin, Fascin 1,Fascin, FLJ38511, FSCN1, HSN, p55, Singed like protein, SNL

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CoraLite® Plus 647-conjugated Fascin Monoclonal an (CL647-66321)

    IF Staining of human tonsillitis using CL647-66321

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite® Plus 647 Fascin antibody (CL647-66321, Clone: 1B5G3 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 647-conjugated Fascin Monoclonal an (CL647-66321)

    FC experiment of HeLa using CL647-66321

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 647 Anti-Human Fascin (CL647-66321, Clone:1B5G3) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF detected inhuman tonsillitis tissue
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-66321 targets Fascin in IF, FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Fascin fusion protein Ag6074
Full Name fascin homolog 1, actin-bundling protein (Strongylocentrotus purpuratus)
Calculated Molecular Weight 55 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberBC000521
Gene Symbol Fascin
Gene ID (NCBI) 6624
RRIDAB_2920280
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

Fascin is a highly conserved and ubiquitous actin cross-linking protein that has a major function in cell motility and adhesion. Fascin localizes to a number of highly dynamic cellular structures that require strong mechanical support which include stress fibers and cellular protrusions such as microvilli, microspikes and lamellipodia. Fascin is up-regulated in many human carcinomas. It's a marker for Reed-Sternberg cells in Hodgkin's disease; it is also a highly selective marker for dendritic cells of lymphoid tissues.

Protocols

Product Specific Protocols
IF protocol for CL Plus 647 Fascin antibody CL647-66321Download protocol
FC protocol for CL Plus 647 Fascin antibody CL647-66321Download protocol
Standard Protocols
Click here to view our Standard Protocols