CoraLite® Plus 488-conjugated Fascin Monoclonal antibody

Fascin Monoclonal Antibody for IF-P, FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IF-P, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1B5G3

Cat no : CL488-66321

Synonyms

FSCN1, Fascin 1,Fascin, FAN1, 55 kDa actin-bundling protein, 55 kDa actin bundling protein

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Validation Data Gallery

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  IF Staining of human tonsillitis using CL488-66321

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CL488-66321 (Fascin antibody) at dilution of 1:100.

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  IF Staining of human tonsillitis using CL488-66321

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CL488-66321 (Fascin antibody) at dilution of 1:100. .

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  FC experiment of SH-SY5Y using CL488-66321

  1X10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human Fascin (CL488-66321, Clone:1B5G3) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL488-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  FC experiment of HeLa using CL488-66321

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human Fascin (CL488-66321, Clone:1B5G3) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL488-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IF-P detected in: human tonsillitis tissue
• Positive FC (Intra) detected in: HeLa cells, SH-SY5Y cells

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66321 targets Fascin in IF-P, FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: Fascin fusion protein Ag6074
• Full Name: fascin homolog 1, actin-bundling protein (Strongylocentrotus purpuratus)
• Calculated Molecular Weight: 55 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC000521
• Gene Symbol: Fascin
• Gene ID (NCBI): 6624
• RRID: AB_2883308
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Fascin is a highly conserved and ubiquitous actin cross-linking protein that has a major function in cell motility and adhesion. Fascin localizes to a number of highly dynamic cellular structures that require strong mechanical support which include stress fibers and cellular protrusions such as microvilli, microspikes and lamellipodia. Fascin is up-regulated in many human carcinomas. It's a marker for Reed-Sternberg cells in Hodgkin's disease; it is also a highly selective marker for dendritic cells of lymphoid tissues.

Related Products

Flow Cytometry Perm Buffer (10X)

PF00011-C