CoraLite® Plus 488-conjugated FXR1 Monoclonal antibody

• Featured Product
• KD/KO Validated

FXR1 Monoclonal Antibody for IF/ICC, IF-P

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF/ICC, IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1E12E8

Cat no : CL488-67813

Synonyms

RNA-binding protein FXR1, hFXR1p, FMR1 autosomal homolog 1, 1E12E8

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Validation Data Gallery

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  IF Staining of mouse brain using CL488-67813

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 488 FXR1 antibody (CL488-67813, Clone: 1E12E8 ) at dilution of 1:200. DAPI (Blue).

• 

  IF Staining of mouse brain using CL488-67813

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 488 FXR1 antibody (CL488-67813, Clone: 1E12E8 ) at dilution of 1:200. DAPI (Blue).

• 

  IF Staining of HeLa using CL488-67813

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 FXR1 antibody (CL488-67813, Clone: 1E12E8 ) at dilution of 1:200.

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Tested Applications

• Positive IF-P detected in: mouse brain tissue
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67813 targets FXR1 in IF/ICC, IF-P applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: FXR1 fusion protein Ag27800
• Full Name: fragile X mental retardation, autosomal homolog 1
• Calculated Molecular Weight: 621 aa, 70 kDa
• Observed Molecular Weight: 70-80 kDa
• GenBank Accession Number: BC028983
• Gene Symbol: FXR1
• Gene ID (NCBI): 8087
• RRID: AB_2919554
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Tumour necrosis factor-alpha (TNF-alpha) is a key mediator of inflammation in host defence against infection and in autoimmune disease. Its production is controlled post-transcriptionally by multiple RNA-binding proteins that interact with the TNF-alpha AU-rich element and regulate its expression; Fragile X mental retardation-related protein 1 (FXR1) is one of these. FXR1 (fragile-X-mental retardation-related protein 1) are RNA-binding proteins that have been demonstrated to impact miRNA-mediated, post-transcriptional gene regulation, and required for embryonic and postnatal development of muscle tissue. It can regulate intracellular transport and local translation of certain mRNAs. The FXR1 exists some isoforms with the MV 69 kDa and 60 kDa.