FXR1 Monoclonal antibody

• Featured Product
• KD/KO Validated

FXR1 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, IP, ELISA

Conjugate

Unconjugated

Publications

2

CloneNo.

1E12E8

Cat no : 67813-1-Ig

Synonyms

RNA-binding protein FXR1, hFXR1p, FMR1 autosomal homolog 1, 1E12E8

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Validation Data Gallery

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  WB analysis using 67813-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67813-1-Ig (FXR1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

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  IP experiment of K-562 using 67813-1-Ig

  IP result of anti-FXR1 (IP:67813-1-Ig, 5ug; Detection:67813-1-Ig 1:1000) with K-562 cells lysate 3680 ug.

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  IHC staining of mouse brain using 67813-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67813-1-Ig (FXR1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 67813-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 67813-1-Ig (FXR1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 67813-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 67813-1-Ig (FXR1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 67813-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67813-1-Ig (FXR1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse brain using 67813-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using FXR1 antibody (67813-1-Ig, Clone: 1E12E8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of HeLa using 67813-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using FXR1 antibody (67813-1-Ig, Clone: 1E12E8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of HeLa using 67813-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using FXR1 antibody (67813-1-Ig, Clone: 1E12E8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells, 4T1 cells
• Positive IP detected in: K-562 cells
• Positive IHC detected in: mouse brain tissue, human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse brain tissue
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below

Product Information

67813-1-Ig targets FXR1 in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: FXR1 fusion protein Ag27800
• Full Name: fragile X mental retardation, autosomal homolog 1
• Calculated Molecular Weight: 621 aa, 70 kDa
• Observed Molecular Weight: 70-80 kDa
• GenBank Accession Number: BC028983
• Gene Symbol: FXR1
• Gene ID (NCBI): 8087
• RRID: AB_2918576
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Tumour necrosis factor-alpha (TNF-alpha) is a key mediator of inflammation in host defence against infection and in autoimmune disease. Its production is controlled post-transcriptionally by multiple RNA-binding proteins that interact with the TNF-alpha AU-rich element and regulate its expression; Fragile X mental retardation-related protein 1 (FXR1) is one of these. FXR1 (fragile-X-mental retardation-related protein 1) are RNA-binding proteins that have been demonstrated to impact miRNA-mediated, post-transcriptional gene regulation, and required for embryonic and postnatal development of muscle tissue. It can regulate intracellular transport and local translation of certain mRNAs. The FXR1 exists some isoforms with the MW 69 kDa and 60 kDa.

Publications

Species	Application	Title
mouse,rat	WB	Br J Pharmacol; Silibinin, a commonly used therapeutic agent for non-alcohol fatty liver disease, functions through upregulating intestinal expression of fibroblast growth factor 15/19; Authors - Yujie Bai
human	WB,IHC	Oncol Lett; FXR1 promotes proliferation, invasion and migration of hepatocellular carcinoma in vitro and in vivo; Authors - Kun Zhao ; KD Validated