FXR1 Monoclonal antibody, PBS Only

FXR1 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, IP, ELISA

Conjugate

Unconjugated

CloneNo.

1E12E8

Cat no : 67813-1-PBS

Synonyms

RNA-binding protein FXR1, hFXR1p, FMR1 autosomal homolog 1, 1E12E8

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Validation Data Gallery

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  WB analysis using 67813-1-Ig (same clone as 67813-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67813-1-Ig (FXR1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 67813-1-PBS in a different storage buffer formulation.

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  IP experiment of K-562 using 67813-1-Ig (same clone as 67813-1-PBS)

  IP result of anti-FXR1 (IP:67813-1-Ig, 5ug; Detection:67813-1-Ig 1:1000) with K-562 cells lysate 3680 ug. This data was developed using the same antibody clone with 67813-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 67813-1-Ig (same clone as 67813-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67813-1-Ig (FXR1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67813-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 67813-1-Ig (same clone as 67813-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 67813-1-Ig (FXR1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67813-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 67813-1-Ig (same clone as 67813-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 67813-1-Ig (FXR1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67813-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 67813-1-Ig (same clone as 67813-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67813-1-Ig (FXR1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67813-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 67813-1-Ig (same clone as 67813-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using FXR1 antibody (67813-1-Ig, Clone: 1E12E8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67813-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67813-1-Ig (same clone as 67813-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using FXR1 antibody (67813-1-Ig, Clone: 1E12E8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67813-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67813-1-Ig (same clone as 67813-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using FXR1 antibody (67813-1-Ig, Clone: 1E12E8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67813-1-PBS in a different storage buffer formulation.

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Product Information

67813-1-PBS targets FXR1 in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: FXR1 fusion protein Ag27800
• Full Name: fragile X mental retardation, autosomal homolog 1
• Calculated Molecular Weight: 621 aa, 70 kDa
• Observed Molecular Weight: 70-80 kDa
• GenBank Accession Number: BC028983
• Gene Symbol: FXR1
• Gene ID (NCBI): 8087
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Tumour necrosis factor-alpha (TNF-alpha) is a key mediator of inflammation in host defence against infection and in autoimmune disease. Its production is controlled post-transcriptionally by multiple RNA-binding proteins that interact with the TNF-alpha AU-rich element and regulate its expression; Fragile X mental retardation-related protein 1 (FXR1) is one of these. FXR1 (fragile-X-mental retardation-related protein 1) are RNA-binding proteins that have been demonstrated to impact miRNA-mediated, post-transcriptional gene regulation, and required for embryonic and postnatal development of muscle tissue. It can regulate intracellular transport and local translation of certain mRNAs. The FXR1 exists some isoforms with the MW 69 kDa and 60 kDa.