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FSHB Monoclonal antibody, PBS Only

FSHB Monoclonal Antibody for IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B10A11

Cat no : 67131-1-PBS

Synonyms

Follitropin beta chain, Follitropin subunit beta, FSH B, FSH beta, FSHB

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human pituitary tissue using FSHB Monoclonal antibody (67131-1-Ig)

    IHC staining of human pituitary using 67131-1-Ig (same clone as 67131-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pituitary tissue slide using 67131-1-Ig (FSHB antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67131-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pituitary tissue using FSHB Monoclonal antibody (67131-1-Ig)

    IHC staining of human pituitary using 67131-1-Ig (same clone as 67131-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pituitary tissue slide using 67131-1-Ig (FSHB antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67131-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pituitary adenoma tissue using FSHB Monoclonal antibody (67131-1-Ig)

    IHC staining of human pituitary adenoma using 67131-1-Ig (same clone as 67131-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pituitary adenoma tissue slide using 67131-1-Ig (FSHB antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67131-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using FSHB Monoclonal antibody (67131-1-Ig)

    IF Staining of HepG2 using 67131-1-Ig (same clone as 67131-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using FSHB antibody (67131-1-Ig, Clone: 1B10A11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67131-1-PBS in a different storage buffer formulation.

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67131-1-PBS targets FSHB in IF, IHC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen FSHB fusion protein Ag13788
Full Name follicle stimulating hormone, beta polypeptide
Calculated Molecular Weight 129 aa, 15 kDa
GenBank Accession NumberBC113488
Gene Symbol FSHB
Gene ID (NCBI) 2488
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

The FSHB gene encodes the beta subunit of follicle-stimulating hormone (FSH), particularly expressed in gonadotroph cells within the anterior pituitary gland and plays a critical role in the regulation of gonadal function, pubertal maturation and reproductive processes in mammals. The transcription of the FSHB gene is critical for the production of the FSH hormone(PMID:28281143). And FSH is required for ovarian folliculogenesis in females, in males it promotes spermatogenesis in conjunction with testosterone(PMID:11739331). Mutations in FSHB gene would result in infertility both in men and women (PMID: 23766128).