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FSHB Monoclonal antibody

FSHB Monoclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1B10A11

Cat no : 67131-1-Ig

Synonyms

Follitropin beta chain, Follitropin subunit beta, FSH B, FSH beta, FSHB

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human pituitary tissue using FSHB Monoclonal antibody (67131-1-Ig)

    IHC staining of human pituitary using 67131-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pituitary tissue slide using 67131-1-Ig (FSHB antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pituitary tissue using FSHB Monoclonal antibody (67131-1-Ig)

    IHC staining of human pituitary using 67131-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pituitary tissue slide using 67131-1-Ig (FSHB antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pituitary adenoma tissue using FSHB Monoclonal antibody (67131-1-Ig)

    IHC staining of human pituitary adenoma using 67131-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pituitary adenoma tissue slide using 67131-1-Ig (FSHB antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using FSHB Monoclonal antibody (67131-1-Ig)

    IF Staining of HepG2 using 67131-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using FSHB antibody (67131-1-Ig, Clone: 1B10A11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive IHC detected inhuman pituitary tissue, human pituitary adenoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67131-1-Ig targets FSHB in IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen FSHB fusion protein Ag13788
Full Name follicle stimulating hormone, beta polypeptide
Calculated Molecular Weight 129 aa, 15 kDa
GenBank Accession NumberBC113488
Gene Symbol FSHB
Gene ID (NCBI) 2488
RRIDAB_2882430
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The FSHB gene encodes the beta subunit of follicle-stimulating hormone (FSH), particularly expressed in gonadotroph cells within the anterior pituitary gland and plays a critical role in the regulation of gonadal function, pubertal maturation and reproductive processes in mammals. The transcription of the FSHB gene is critical for the production of the FSH hormone(PMID:28281143). And FSH is required for ovarian folliculogenesis in females, in males it promotes spermatogenesis in conjunction with testosterone(PMID:11739331). Mutations in FSHB gene would result in infertility both in men and women (PMID: 23766128).

Protocols

Product Specific Protocols
IHC protocol for FSHB antibody 67131-1-IgDownload protocol
IF protocol for FSHB antibody 67131-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols