FOXP4 Recombinant antibody

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  IF Staining of HepG2 using 84855-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using FOXP4 antibody (84855-1-RR, Clone: 242356B4 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  IF Staining of HeLa using 84855-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using FOXP4 antibody (84855-1-RR, Clone: 242356B4 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  FC experiment of HepG2 using 84855-1-RR

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug FOXP4 Recombinant antibody (84855-1-RR, Clone:242356B4) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  Affinity and Kinetic Characterization of 84855-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84855-1-RR against Human FOXP4 were performed. The affinity constant is 0.402 nM.

FOXP4 {ptg:Brand} Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Cat No. 84855-1-RR

Clone No.242356B4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Synonyms

Fork head-related protein-like A, FKHLA, 242356B4

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Tested Applications

• Positive IF/ICC detected in: HeLa cells, HepG2 cells
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84855-1-RR targets FOXP4 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: FOXP4 fusion protein Ag10047
• Full Name: forkhead box P4
• Calculated Molecular Weight: 678 aa, 73 kDa
• GenBank Accession Number: BC052803
• Gene Symbol: FOXP4
• Gene ID (NCBI): 116113
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q8IVH2
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FOXP4, also named as FKHLA, belongs to the FOX family. FOXP4 has been implicated in diverse biological processes in tumor initiation and progression. It has been demonstrated that FOXP4 is significantly upregulated in breast cancer, hepatocellular carcinoma and oral squamous cell carcinoma (PMID: 34590150). FOXP4 has 3 isoforms with the molecular mass of 72-73 kDa.