FOXP3 Monoclonal antibody, PBS Only (Capture)

FOXP3 Monoclonal Antibody for IHC, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IHC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2A1E7

Cat no : 68803-2-PBS

Synonyms

Forkhead box protein P3 41 kDa form, Forkhead box protein P3, forkhead box P3, DIETER, AIID

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Validation Data Gallery

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  IHC staining of human ovary cancer using 68803-2-Ig (same clone as 68803-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 68803-2-Ig (FOXP3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68803-2-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 68803-2-Ig (same clone as 68803-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 68803-2-Ig (FOXP3 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68803-2-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 68803-2-Ig (same clone as 68803-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 68803-2-Ig (FOXP3 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68803-2-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 68803-2-Ig (same clone as 68803-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 68803-2-Ig (FOXP3 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68803-2-PBS in a different storage buffer formulation.

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  IHC staining of human rectal cancer using 68803-2-Ig (same clone as 68803-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 68803-2-Ig (FOXP3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68803-2-PBS in a different storage buffer formulation.

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  IHC staining of MJ using 68803-2-Ig (same clone as 68803-2-PBS)

  Immunohistochemical analysis of paraffin-embedded MJ cells slide using 68803-2-Ig (FOXP3 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68803-2-PBS in a different storage buffer formulation.

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  IHC staining of MJ using 68803-2-Ig (same clone as 68803-2-PBS)

  Immunohistochemical analysis of paraffin-embedded MJ cells slide using 68803-2-Ig (FOXP3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68803-2-PBS in a different storage buffer formulation.

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  IHC staining of human appendicitis using 68803-2-Ig (same clone as 68803-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 68803-2-Ig (FOXP3 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68803-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human appendicitis using 68803-2-Ig (same clone as 68803-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 68803-2-Ig (FOXP3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68803-2-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 68803-2-Ig (same clone as 68803-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68803-2-Ig (FOXP3 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68803-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 68803-2-Ig (same clone as 68803-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68803-2-Ig (FOXP3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68803-2-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 68803-2-Ig (same clone as 68803-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 68803-2-Ig (FOXP3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68803-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human rectal cancer using 68803-2-Ig (same clone as 68803-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 68803-2-Ig (FOXP3 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68803-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50216-1

  Cytometric bead array standard curve of MP50216-1, FOXP3 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68803-2-PBS. Detection antibody: 68838-1-PBS. Standard:Ag17571. Range: 0.781-100 ng/mL.

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Product Information

68803-2-PBS targets FOXP3 as part of a matched antibody pair:

MP50216-1: 68803-2-PBS capture and 68838-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: FOXP3 fusion protein Ag23089
• Full Name: forkhead box P3
• Calculated Molecular Weight: 431 aa, 47 kDa
• GenBank Accession Number: BC113401
• Gene Symbol: FOXP3
• Gene ID (NCBI): 50943
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

FOXP3, also named as IPEX, JM2 and Scurfin, is a transcription factor. POXP3 plays a critical role in the control of immune response. Defects in FOXP3 are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX) which also known as X-linked autoimmunity-immunodeficiency syndrome.