FOXP3 Monoclonal antibody, PBS Only (Capture)

FOXP3 Monoclonal Antibody for WB, IHC, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2A1E7

Cat no : 68803-2-PBS

Synonyms

Forkhead box protein P3 41 kDa form, Forkhead box protein P3, forkhead box P3, DIETER, AIID

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Validation Data Gallery

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  WB analysis of MJ using 68803-2-Ig (same clone as 68803-2-PBS)

  MJ cells were subjected to SDS PAGE followed by western blot with 68803-2-Ig (FOXP3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68803-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary cancer using 68803-2-Ig (same clone as 68803-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 68803-2-Ig (FOXP3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68803-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 68803-2-Ig (same clone as 68803-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 68803-2-Ig (FOXP3 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68803-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 68803-2-Ig (same clone as 68803-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 68803-2-Ig (FOXP3 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68803-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary cancer using 68803-2-Ig (same clone as 68803-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 68803-2-Ig (FOXP3 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68803-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human appendicitis using 68803-2-Ig (same clone as 68803-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 68803-2-Ig (FOXP3 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68803-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human appendicitis using 68803-2-Ig (same clone as 68803-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 68803-2-Ig (FOXP3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68803-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 68803-2-Ig (same clone as 68803-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68803-2-Ig (FOXP3 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68803-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 68803-2-Ig (same clone as 68803-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68803-2-Ig (FOXP3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68803-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human lymphoma using 68803-2-Ig (same clone as 68803-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 68803-2-Ig (FOXP3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68803-2-PBS in a different storage buffer formulation.

• 

  IHC staining of MJ using 68803-2-Ig (same clone as 68803-2-PBS)

  Immunohistochemical analysis of paraffin-embedded MJ cells slide using 68803-2-Ig (FOXP3 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68803-2-PBS in a different storage buffer formulation.

• 

  IHC staining of MJ using 68803-2-Ig (same clone as 68803-2-PBS)

  Immunohistochemical analysis of paraffin-embedded MJ cells slide using 68803-2-Ig (FOXP3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68803-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human rectal cancer using 68803-2-Ig (same clone as 68803-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 68803-2-Ig (FOXP3 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68803-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human rectal cancer using 68803-2-Ig (same clone as 68803-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 68803-2-Ig (FOXP3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68803-2-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP50216-1

  Cytometric bead array standard curve of MP50216-1, FOXP3 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68803-2-PBS. Detection antibody: 68838-1-PBS. Standard:Ag17571. Range: 0.781-100 ng/mL.

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Product Information

68803-2-PBS targets FOXP3 as part of a matched antibody pair:

MP50216-1: 68803-2-PBS capture and 68838-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: FOXP3 fusion protein Ag23089
• Full Name: forkhead box P3
• Calculated Molecular Weight: 431 aa, 47 kDa
• Observed Molecular Weight: 47 kDa
• GenBank Accession Number: BC113401
• Gene Symbol: FOXP3
• Gene ID (NCBI): 50943
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

FOXP3 is a transcription factor belonging to the Forkhead box (FOX) protein family. It contains a forkhead-winged helix domain that acts as a transcriptional activator or repressor. FOXP3 is the master regulator of regulatory T cells (Tregs), a subset of CD4+ T cells with potent suppressive activity. It plays a critical role in maintaining self-tolerance and preventing autoimmune diseases by controlling the development and function of Tregs. The expression of FOXP3 is quite limited, and the cellular taxa present in the tissue are relatively low. Therefore, if you intend to perform Western blotting (WB), it is advisable to enrich the target cells prior to detection.