FOXP3 Monoclonal antibody

FOXP3 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2A1E7

Cat no : 68803-2-Ig

Synonyms

Forkhead box protein P3 41 kDa form, Forkhead box protein P3, forkhead box P3, DIETER, AIID

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Validation Data Gallery

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  WB analysis of MJ using 68803-2-Ig

  MJ cells were subjected to SDS PAGE followed by western blot with 68803-2-Ig (FOXP3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human ovary cancer using 68803-2-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 68803-2-Ig (FOXP3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 68803-2-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 68803-2-Ig (FOXP3 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 68803-2-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 68803-2-Ig (FOXP3 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of MJ using 68803-2-Ig

  Immunohistochemical analysis of paraffin-embedded MJ cells slide using 68803-2-Ig (FOXP3 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of MJ using 68803-2-Ig

  Immunohistochemical analysis of paraffin-embedded MJ cells slide using 68803-2-Ig (FOXP3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human appendicitis using 68803-2-Ig

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 68803-2-Ig (FOXP3 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human appendicitis using 68803-2-Ig

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 68803-2-Ig (FOXP3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 68803-2-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68803-2-Ig (FOXP3 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 68803-2-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68803-2-Ig (FOXP3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 68803-2-Ig

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 68803-2-Ig (FOXP3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human rectal cancer using 68803-2-Ig

  Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 68803-2-Ig (FOXP3 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human rectal cancer using 68803-2-Ig

  Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 68803-2-Ig (FOXP3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary cancer using 68803-2-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 68803-2-Ig (FOXP3 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: MJ cells
• Positive IHC detected in: human ovary cancer tissue, human appendicitis tissue, human lung cancer tissue, human lymphoma tissue, human rectal cancer tissue, human tonsillitis tissue, MJ cells; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68803-2-Ig targets FOXP3 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: FOXP3 fusion protein Ag23089
• Full Name: forkhead box P3
• Calculated Molecular Weight: 431 aa, 47 kDa
• Observed Molecular Weight: 47 kDa
• GenBank Accession Number: BC113401
• Gene Symbol: FOXP3
• Gene ID (NCBI): 50943
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FOXP3 is a transcription factor belonging to the Forkhead box (FOX) protein family. It contains a forkhead-winged helix domain that acts as a transcriptional activator or repressor. FOXP3 is the master regulator of regulatory T cells (Tregs), a subset of CD4+ T cells with potent suppressive activity. It plays a critical role in maintaining self-tolerance and preventing autoimmune diseases by controlling the development and function of Tregs. The expression of FOXP3 is quite limited, and the cellular taxa present in the tissue are relatively low. Therefore, if you intend to perform Western blotting (WB), it is advisable to enrich the target cells prior to detection.