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FOXP3 Monoclonal antibody

FOXP3 Monoclonal Antibody for IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2A1E7

Cat no : 68803-2-Ig

Synonyms

Forkhead box protein P3 41 kDa form, Forkhead box protein P3, forkhead box P3, DIETER, AIID

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using FOXP3 Monoclonal antibody (68803-2-Ig)

    IHC staining of human ovary cancer using 68803-2-Ig

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 68803-2-Ig (FOXP3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using FOXP3 Monoclonal antibody (68803-2-Ig)

    IHC staining of human tonsillitis using 68803-2-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 68803-2-Ig (FOXP3 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using FOXP3 Monoclonal antibody (68803-2-Ig)

    IHC staining of human tonsillitis using 68803-2-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 68803-2-Ig (FOXP3 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using FOXP3 Monoclonal antibody (68803-2-Ig)

    IHC staining of human lymphoma using 68803-2-Ig

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 68803-2-Ig (FOXP3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human rectal cancer tissue using FOXP3 Monoclonal antibody (68803-2-Ig)

    IHC staining of human rectal cancer using 68803-2-Ig

    Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 68803-2-Ig (FOXP3 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human rectal cancer tissue using FOXP3 Monoclonal antibody (68803-2-Ig)

    IHC staining of human rectal cancer using 68803-2-Ig

    Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 68803-2-Ig (FOXP3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using FOXP3 Monoclonal antibody (68803-2-Ig)

    IHC staining of human ovary cancer using 68803-2-Ig

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 68803-2-Ig (FOXP3 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human appendicitis tissue using FOXP3 Monoclonal antibody (68803-2-Ig)

    IHC staining of human appendicitis using 68803-2-Ig

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 68803-2-Ig (FOXP3 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human appendicitis tissue using FOXP3 Monoclonal antibody (68803-2-Ig)

    IHC staining of human appendicitis using 68803-2-Ig

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 68803-2-Ig (FOXP3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using FOXP3 Monoclonal antibody (68803-2-Ig)

    IHC staining of human lung cancer using 68803-2-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68803-2-Ig (FOXP3 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using FOXP3 Monoclonal antibody (68803-2-Ig)

    IHC staining of human lung cancer using 68803-2-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68803-2-Ig (FOXP3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of MJ cells using FOXP3 Monoclonal antibody (68803-2-Ig)

    IHC staining of MJ using 68803-2-Ig

    Immunohistochemical analysis of paraffin-embedded MJ cells slide using 68803-2-Ig (FOXP3 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of MJ cells using FOXP3 Monoclonal antibody (68803-2-Ig)

    IHC staining of MJ using 68803-2-Ig

    Immunohistochemical analysis of paraffin-embedded MJ cells slide using 68803-2-Ig (FOXP3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive IHC detected inhuman ovary cancer tissue, human appendicitis tissue, human lung cancer tissue, human lymphoma tissue, human rectal cancer tissue, human tonsillitis tissue, MJ cells
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68803-2-Ig targets FOXP3 in IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen FOXP3 fusion protein Ag23089
Full Name forkhead box P3
Calculated Molecular Weight 431 aa, 47 kDa
GenBank Accession NumberBC113401
Gene Symbol FOXP3
Gene ID (NCBI) 50943
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FOXP3, also named as IPEX, JM2 and Scurfin, is a transcription factor. POXP3 plays a critical role in the control of immune response. Defects in FOXP3 are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX) which also known as X-linked autoimmunity-immunodeficiency syndrome.

Protocols

Product Specific Protocols
IHC protocol for FOXP3 antibody 68803-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols