CoraLite® Plus 750-conjugated FOXO1 Recombinant antibody

FOXO1 Recombinant Antibody for WB

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB

Conjugate

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

230183D1

Cat no : CL750-82997

Synonyms

FKH1, FKHR, forkhead box O1, Forkhead box protein O1, Forkhead box protein O1A, Forkhead in rhabdomyosarcoma, FOXO1, FOXO1A

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Validation Data Gallery

• 

  WB analysis using CL750-82997

  Various lysates were subjected to SDS PAGE followed by western blot with CL750-82997 (FOXO1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HEK-293 cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL750-82997 targets FOXO1 in WB applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: FOXO1 fusion protein Ag13296
• Full Name: forkhead box O1
• Calculated Molecular Weight: 655 aa, 70 kDa
• Observed Molecular Weight: 70-80 kDa
• GenBank Accession Number: BC021981
• Gene Symbol: FOXO1
• Gene ID (NCBI): 2308
• Conjugate: CoraLite® Plus 750 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 755 nm / 780 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

FOXO1, also named as FOXO1A, FKHR and FKH1, is a member of the FOXO subfamily of Forkhead transcription factors. FOXO1 is a transcription factor which acts as a regulator of cell responses to oxidative stress. FOXO1 interacts with LRPPRC and SIRT1. In the presence of KIRT1, FOXO1 mediates down-regulation of cyclin D1 and up-regulation of CDKN1B levels which are required for cell transition from proliferative growth to quiescence. FOXO1 contains three predicted protein kinase B phosphorylation sites (Thr-24, Ser-256, and Ser-319) that are conserved in other FOXO proteins. The t(2;13) and the variant t(1;13) translocations generate PAX3/FKHR and PAX7/FKHR fusion proteins respectively. The resulting protein is a transcriptional activator. Defects in FOXO1 are a cause of rhabdomyosarcoma type 2 (RMS2).