Tested Applications
| Positive IF/ICC detected in | A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-83735-4 targets FOXK2 in IF/ICC applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | FOXK2 fusion protein Ag33625 Predict reactive species |
| Full Name | forkhead box K2 |
| Calculated Molecular Weight | 69 kDa |
| Observed Molecular Weight | 69-79 kDa |
| GenBank Accession Number | NM_004514.3 |
| Gene Symbol | FOXK2 |
| Gene ID (NCBI) | 3607 |
| RRID | AB_3673298 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q01167 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
FOXK2 (forkhead box K2), also known as ILF and ILF1. FOXK2 is localized at nucleus and cytoplasm. It is also expected to be expressed in testis, brain and lymphoid and non-lymphoid cells (PMID: 1339390). The protein encoded by this gene contains a fork head DNA binding domain. This protein can bind to the purine-rich motifs of the HIV long terminal repeat (LTR), and to the similar purine-rich motif in the interleukin 2 (IL2) promoter. It may be involved in the regulation of viral and cellular promoter elements. And its molecular weight is 69 kDa. Anett Marais demonstrate that FOXK2 is phosphorylated in a cell cycle-dependent manner. This phosphorylation peaks during M phase and is mediated by CDK·cyclin complexes. FOXK2 exhibits a periodic rise in its phosphorylation levels during the cell cycle, with hyperphosphorylation occurring in mitotic cells (PMID: 20810654).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 FOXK2 antibody CL488-83735-4 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
