FOXK2 Recombinant antibody

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  WB analysis using 83735-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83735-4-RR (FOXK2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 83735-4-RR

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83735-4-RR (FOXK2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 83735-4-RR

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using FOXK2 antibody (83735-4-RR, Clone: 240692A5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  IF Staining of A431 using 83735-4-RR

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using FOXK2 antibody (83735-4-RR, Clone: 240692A5 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  FC experiment of SH-SY5Y using 83735-4-RR

  1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug FOXK2 Recombinant antibody (83735-4-RR, Clone:240692A5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  FC experiment of HEK-293T using 83735-4-RR

  1x10^6 HEK-293T cells were intracellularly stained with 0.25 ug FOXK2 Recombinant antibody (83735-4-RR, Clone:240692A5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  Affinity and Kinetic Characterization of 83735-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83735-4-RR against Human FOXK2 were performed. The affinity constant is 0.119 nM.

FOXK2 Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Cat No. 83735-4-RR

Clone No.240692A5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Synonyms

ILF1, ILF 1, ILF, Forkhead box protein K2, forkhead box K2

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Tested Applications

• Positive WB detected in: HEK-293T cells, HeLa cells, MCF-7 cells, T-47D cells, MDA-MB-231 cells, U2OS cells
• Positive IHC detected in: human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A431 cells
• Positive FC (Intra) detected in: SH-SY5Y cells, HEK-293T cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83735-4-RR targets FOXK2 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: FOXK2 fusion protein Ag33625
• Full Name: forkhead box K2
• Calculated Molecular Weight: 69 kDa
• Observed Molecular Weight: 69-79 kDa
• GenBank Accession Number: NM_004514.3
• Gene Symbol: FOXK2
• Gene ID (NCBI): 3607
• RRID: AB_3671335
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q01167
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FOXK2 (forkhead box K2), also known as ILF and ILF1. FOXK2 is localized at nucleus and cytoplasm. It is also expected to be expressed in testis, brain and lymphoid and non-lymphoid cells (PMID: 1339390). The protein encoded by this gene contains a fork head DNA binding domain. This protein can bind to the purine-rich motifs of the HIV long terminal repeat (LTR), and to the similar purine-rich motif in the interleukin 2 (IL2) promoter. It may be involved in the regulation of viral and cellular promoter elements. And its molecular weight is 69 kDa. Anett Marais demonstrate that FOXK2 is phosphorylated in a cell cycle-dependent manner. This phosphorylation peaks during M phase and is mediated by CDK·cyclin complexes. FOXK2 exhibits a periodic rise in its phosphorylation levels during the cell cycle, with hyperphosphorylation occurring in mitotic cells (PMID: 20810654).