FOXC1 Polyclonal antibody, PBS Only

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  WB analysis of HEK-293 using 55365-1-AP (same clone as 55365-1-PBS)

  WB result of FOXC1 antibody (55365-1-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-FOXC1 transfected HEK-293 cells. This data was developed using the same antibody clone with 55365-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 55365-1-AP (same clone as 55365-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 55365-1-AP (FOXC1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55365-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 55365-1-AP (same clone as 55365-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 55365-1-AP (FOXC1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55365-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 55365-1-AP (same clone as 55365-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 55365-1-AP (FOXC1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55365-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 55365-1-AP (same clone as 55365-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 55365-1-AP (FOXC1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55365-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HepG2 using 55365-1-AP (same clone as 55365-1-PBS)

  IP result of anti-FOXC1 (IP:55365-1-AP, 4ug; Detection:55365-1-AP 1:300) with HepG2 cells lysate 4000 ug. This data was developed using the same antibody clone with 55365-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

FOXC1 Polyclonal Antibody for WB, IP, Indirect ELISA

Cat No. 55365-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, Indirect ELISA

Synonyms

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Product Information

55365-1-PBS targets FOXC1 in WB, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: forkhead box C1
• Calculated Molecular Weight: 57 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: NM_001453
• Gene Symbol: FOXC1
• Gene ID (NCBI): 2296
• RRID: AB_2881316
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q12948
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

FOXC1, also named as FKHL7 and FREAC3, binding of FREAC-3 and FREAC-4 to their cognate sites results in bending of the DNA at an angle of 80-90 degrees. Defects in FOXC1 are the cause of Axenfeld-Rieger syndrome type 3 (RIEG3). Defects in FOXC1 are the cause of iridogoniodysgenesis anomaly (IGDA). Defects in FOXC1 are a cause of Peters anomaly. This antibody is specific to FOXC1. Phosphorylation modification of Foxc1 protein may be responsible for the larger molecular weight of detection compared with theoretical molecular weight (PMID:27708239;16403239).