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- KD/KO Validated
FOXC1 Polyclonal antibody
FOXC1 Polyclonal Antibody for WB, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IHC, IP, CoIP, ELISA, IF
Conjugate
Unconjugated
Cat no : 55365-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | MCF-7 cells, HEK-293 cells, HepG2 cells |
| Positive IP detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 5 publications below |
| IHC | See 2 publications below |
| IF | See 1 publications below |
| CoIP | See 1 publications below |
Product Information
55365-1-AP targets FOXC1 in WB, IHC, IP, CoIP, ELISA, IF applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide |
| Full Name | forkhead box C1 |
| Calculated Molecular Weight | 57 kDa |
| Observed Molecular Weight | 70 kDa |
| GenBank Accession Number | NM_001453 |
| Gene Symbol | FOXC1 |
| Gene ID (NCBI) | 2296 |
| RRID | AB_2881316 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
FOXC1, also named as FKHL7 and FREAC3, binding of FREAC-3 and FREAC-4 to their cognate sites results in bending of the DNA at an angle of 80-90 degrees. Defects in FOXC1 are the cause of Axenfeld-Rieger syndrome type 3 (RIEG3). Defects in FOXC1 are the cause of iridogoniodysgenesis anomaly (IGDA). Defects in FOXC1 are a cause of Peters anomaly. This antibody is specific to FOXC1. Phosphorylation modification of Foxc1 protein may be responsible for the larger molecular weight of detection compared with theoretical molecular weight (PMID:27708239;16403239).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for FOXC1 antibody 55365-1-AP | Download protocol |
| IP protocol for FOXC1 antibody 55365-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Bone The osteoporosis risk variant rs9820407 at 3p22.1 acts as an allele-specific enhancer to regulate CTNNB1 expression by long-range chromatin loop formation.
| ||
Tumour Biol The long noncoding RNA FOXCUT promotes proliferation and migration by targeting FOXC1 in nasopharyngeal carcinoma.
| ||
Mol Med Rep FOXC1 silencing inhibits the epithelial‑to‑mesenchymal transition of glioma cells: Involvement of β‑catenin signaling.
| ||
Phytomedicine Icaritin inhibits endometrial carcinoma cells by suppressing O-GlcNAcylation of FOXC1 | ||
J Oral Pathol Med Single-cell RNA sequencing reveals tumor heterogeneity within salivary gland pleomorphic adenoma: A preliminary study | ||
Phytomedicine Berberine promotes lacteal junction zippering and ameliorates diet-induced obesity through the RhoA/ROCK signaling pathway |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Sarah (Verified Customer) (08-20-2021) | I was sent 4 antibodies to try. You can see that JUN (1:1000) and NFKB1 (1:1000) worked, but FOXC1 (1:300) and HES1 (1:500) did not. I have shown that the cells express the mRNA for these proteins by qPCR.
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