FNDC5 Recombinant antibody, PBS Only

FNDC5 Uni-rAb^TM Recombinant Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat, pig

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2H3

Cat no : 82671-1-PBS

Synonyms

FNDC5, FRCP2

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Validation Data Gallery

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  WB analysis using 82671-1-RR (same clone as 82671-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82671-1-RR (FNDC5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82671-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse skeletal muscle using 82671-1-RR (same clone as 82671-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 82671-1-RR (FNDC5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82671-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse skeletal muscle using 82671-1-RR (same clone as 82671-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 82671-1-RR (FNDC5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82671-1-PBS in a different storage buffer formulation.

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Product Information

82671-1-PBS targets FNDC5 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

• Tested Reactivity: Human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: FNDC5 fusion protein Ag29714
• Full Name: fibronectin type III domain containing 5
• Calculated Molecular Weight: 212 aa, 24 kDa
• Observed Molecular Weight: 25-30 kDa
• GenBank Accession Number: BC062297
• Gene Symbol: FNDC5
• Gene ID (NCBI): 252995
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

fibronectin type III domain containing 5(FNDC5) encodes 23 kDa protein, which is a membrane protein that is cleaved and secreted as a newly identified hormone, irisin. Exercise-induced FNDC5 gene expression in muscles was accompanied by a parallel increase in the concentration of circulating irisin, which in turn activates adipocyte thermogenic programs, leading to mitochondrial heat production and energy expenditure.